Rat Transitional Endoplasmic Reticulum ATPase (VCP) ELISA Kit | VCP elisa kit

Rat Transitional Endoplasmic Reticulum ATPase (VCP) ELISA Kit

Cat. Num. AAA9711613

• Reactivity: Rat
• Synonyms: Transitional Endoplasmic Reticulum ATPase (VCP); Rat Transitional Endoplasmic Reticulum ATPase (VCP) ELISA Kit; VCP; IBMPFD; MGC131997; MGC148092; MGC8560; TERA; p97; transitional endoplasmic reticulum ATPase; yeast Cdc48p homolog; VCP elisa kit

• Reactivity: Rat
• Specificity: Rat Transitional endoplasmic reticulum ATPase (VCP) ELISA Kit has high sensitivity and excellent specificity for detection of Rat VCP. No significant cross-reactivity or interference between Rat VCP and analogues was observed.
• Sequence Length: 806

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Rat Transitional endoplasmic reticulum ATPase (VCP) Standard Curve.)

Related Product Information for VCP elisa kit

Description: This Rat Transitional endoplasmic reticulum ATPase (VCP) ELISA Kit employs a two-site sandwich ELISA to quantitate VCP in samples. An antibody specific for VCP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VCP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VCP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VCP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: The deduced protein contains 1,236 amino acids. RT-PCR ELISA detected moderate VCPIP1 expression in all adult and fetal tissues examined.Reassembly of the Golgi apparatus from membrane fragments after cell division requires the ATPases NSF and p97 (VCP), as well as the cofactor p47 (NSFL1C) and the receptor syntaxin-5 (STX5A). rat Vcip135 was essential for p97/p47-mediated membrane fusion and that Vcip135 bound to the p97/p47/Stx5a complex and dissociated it via p97-catalyzed ATP hydrolysis. Microinjection of antibodies against Vcip135 and p47 in rat kidney cells showed that Vcip135 and p47 were required for Golgi and endoplasmic reticulum assembly.a cycle of ubiquitination and VCIP135-mediated deubiquitination regulates Golgi membrane dynamics during mitosis.

NCBI and Uniprot Product Information

• NCBI GI #: 17865351
• NCBI GeneID: 116643
• NCBI Accession #: NP_446316.1
• NCBI GenBank Nucleotide #: NP_446316.1
• UniProt Accession #: P46462
• NCBI Official Full Name: transitional endoplasmic reticulum ATPase
• NCBI Official Synonym Full Names: valosin-containing protein
• NCBI Official Symbol: Vcp
• NCBI Protein Information: transitional endoplasmic reticulum ATPase
• UniProt Protein Name: Transitional endoplasmic reticulum ATPase
• Protein Family: Transitional endoplasmic reticulum ATPase
• UniProt Gene Name: Vcp
• UniProt Synonym Gene Names: TER ATPase; VCP

NCBI Description

ATP binding protein involved in vesicle transport and fusion; important for proper functioning of the endoplasmic reticulum [RGD, Feb 2006]

Uniprot Description

Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Required for cytoplasmic retrotranslocation of stressed/damaged mitochondrial outer-membrane proteins and their subsequent proteasomal degradation. Essential for the maturation of ubiquitin-containing autophagosomes and the clearance of ubiquitinated protein by autophagy.