Western Blot (WB)
(Western Blot detection against Immunogen (88kD).)
Mouse anti-Human AF9 Monoclonal Antibody | anti-AF9 antibody
AF9 (MLLT3, AF9, YEATS3, Protein AF-9, ALL1-fused Gene from Chromosome 9 Protein, Myeloid/Lymphoid or Mixed-lineage Leukemia Translocated to Chromosome 3 Protein, YEATS Domain-containing Protein 3, FLJ2035) (HRP)
Gene Names
MLLT3; AF9; YEATS3
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
AF9; Monoclonal Antibody; AF9 (MLLT3; YEATS3; Protein AF-9; ALL1-fused Gene from Chromosome 9 Protein; Myeloid/Lymphoid or Mixed-lineage Leukemia Translocated to Chromosome 3 Protein; YEATS Domain-containing Protein 3; FLJ2035) (HRP); anti-AF9 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3C11
Specificity
Recognizes human MLLT3.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Applicable Applications for anti-AF9 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-568 from human MLLT3 (AAH36089) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MASSCAVQVKLELGHRAQVRKKPTVEGFTHDWMVFVRGPEHSNIQHFVEKVVFHLHESFPRPKRVCKDPPYKVEESGYAGFILPIEVYFKNKEEPRKVRFDYDLFLHLEGHPPVNHLRCEKLTFNNPTEDFRRKLLKAGGDPNRSIHTSSSSSSSSSSSSSSSSSSSSSSSSGSSSSSSSSSSSSSSSSSTSFSKPHKLMKEHKEKPSKDSREHKSAFKEPSRDHNKSSKESSKKPKENKPLKEEKIVPKMAFKEPKPMSKEPKPDSNLLTITSGQDKKAPSKRPPISDSEELSAKKRKKSSSEALFKSFSSAPPLILTCSADKKQIKDKSHVKMGKVKIESETSEKKKSTLPPFDDIVDPNDSDVEENISSKSDSEQPSPASSSSSSSSSFTPSQTRQQGPLRSIMKDLHSDDNEEESDEVEDNDNDSEMERPVNRGGSRSRRVSLSDGSDSESSSASSPLHHEPPPPLLKTNNNQILEVKSPIKQSKSDKQIKNGECDKAYLDELVELHRRLMTLRERHILQQIVNLIEETGHFHITNTTFDFDLCSLDKTTVRKLQSYLETSGTS
Conjugate
HRP
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (88kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (88kD).)
Testing Data
(Detection limit for recombinant GST tagged MLLT3 is ~3ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged MLLT3 is ~3ng/ml as a capture antibody.)
Product Categories/Family for anti-AF9 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
63,106 Da
NCBI Official Full Name
Homo sapiens myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3, mRNA
NCBI Official Synonym Full Names
MLLT3, super elongation complex subunit
NCBI Official Symbol
MLLT3
NCBI Official Synonym Symbols
AF9; YEATS3
NCBI Protein Information
protein AF-9
UniProt Protein Name
Protein AF-9
UniProt Gene Name
MLLT3
UniProt Synonym Gene Names
AF9; YEATS3
UniProt Entry Name
AF9_HUMAN
Uniprot Description
MLLT3: A chromosomal aberration involving MLLT3 is associated with acute leukemias. Translocation t(9;11)(p22;q23) with MLL/HRX. The result is a rogue activator protein.
Protein type: Cell development/differentiation; Oncoprotein
Chromosomal Location of Human Ortholog: 9p22
Cellular Component: transcription elongation factor complex; nucleus
Molecular Function: protein binding
Biological Process: anterior/posterior pattern formation; segment specification; regulation of transcription, DNA-dependent; transcription, DNA-dependent
Research Articles on AF9
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Product Notes
The AF9 mllt3 (Catalog #AAA6151118) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AF9 (MLLT3, AF9, YEATS3, Protein AF-9, ALL1-fused Gene from Chromosome 9 Protein, Myeloid/Lymphoid or Mixed-lineage Leukemia Translocated to Chromosome 3 Protein, YEATS Domain-containing Protein 3, FLJ2035) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AF9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AF9 mllt3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AF9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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