Mouse p44/42 MAPK Monoclonal Antibody | anti-p44/42 MAPK antibody

Anti-p44/42 MAPK (Erk1/2) Mouse mAb

Cat. Num. AAA475392

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified
• Synonyms: p44/42 MAPK; Monoclonal Antibody; Anti-p44/42 MAPK (Erk1/2) Mouse mAb; ERK 1; ERK 2; ERK-2; ERK1; erk1/2; ERK2; ERT1; ERT2; Extracellular signal regulated kinase 1; Extracellular signal regulated kinase 2; Extracellular signal-regulated kinase 2; HS44KDAP; HUMKER1A; Insulin stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; MAPK1; MAPK2; MAPK3; MGC20180; Microtubule associated protein 2 kinase; Mitogen activated protein kinase 1; Mitogen activated protein kinase 2; Mitogen activated protein kinase 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; MK01_HUMAN; p38; p40; p41; p41mapk; p42 MAPK; p42-MAPK; p42MAPK; p44 ERK1; p44 MAPK; p44ERK1; p44MAPK; PRKM 1; PRKM 2; PRKM 3; PRKM1; PRKM2; PRKM3; Protein kinase mitogen activated 1; Protein kinase mitogen activated 2; Protein kinase mitogen activated 3; Protein tyrosine kinase ERK 2; anti-p44/42 MAPK antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: lgG1
• Clone Number: 4F3-A10-E7
• Purity/Purification: Affinity Purified
• Form/Format: Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
• Concentration: 7.21mg/ml (varies by lot)

• Applicable Applications for anti-p44/42 MAPK antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Immunogen: Human p44 MAPK (Erk1) synthetic peptide conjugated to KLH.
• Sensitivity: This antibody detects endogenous levels of p44/42 MAPK (Erk1/2) and does not cross-react with related proteins.
• Sequence Similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
• Subunit Structure: ERK1: Binds both upstream activators and downstream substrates in multimolecular complexes. Found in a complex with at least BRAF, HRAS, MAP2K1/MEK1, MAPK3 and RGS14 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with ADAM15, ARRB2, CANX, DAPK1 (via death domain), HSF4, IER3, MAP2K1/MEK1, MORG1, NISCH, and SGK1. Interacts with PEA15 and MKNK2 (By similarity). MKNK2 isoform 1 binding prevents from dephosphorylation and inactivation (By similarity). Interacts with TPR. Interacts with CDKN2AIP. ERK2: Binds both upstream activators and downstream substrates in multimolecular complexes. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with ADAM15, ARHGEF2, ARRB2, DAPK1 (via death domain), HSF4, IER3, IPO7, DUSP6, NISCH, SGK1, and isoform 1 of NEK2. Interacts (phosphorylated form) with CAV2 ('Tyr-19'-phosphorylated form); the interaction, promoted by insulin, leads to nuclear location and MAPK1 activation. Interacts with MORG1, PEA15 and MKNK2 (By similarity). MKNK2 isoform 1 binding prevents from dephosphorylation and inactivation (By similarity). Interacts with DCC (By similarity). The phosphorylated form interacts with PML (isoform PML-4). Interacts with STYX.
• Subcellular Location: Nucleus.

Western Blot (WB)

(Western blot analysis of extracts from Jurkat, A549, C6, 3T3 and Hela cell lysates using p44/42 MAPK (Erk1/2) mouse mAb (1:1000 diluted).Predicted band size:42/44KDa.Observed band size:42/44KDa.)

Related Product Information for anti-p44/42 MAPK antibody

Entrez Summary: This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.