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Western Blot (WB) (Western blot analysis of p16 ARC on different cell lysates using anti-p16 ARC antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: SK-BR-3)

Rabbit p16 ARC Monoclonal Antibody | anti-ARPC5 antibody

p16 ARC Antibody

Gene Names
ARPC5; ARC16; p16-Arc; dJ127C7.3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
p16 ARC; Monoclonal Antibody; p16 ARC Antibody; Actin related protein 2/3 complex subunit 5 (16 kD) antibody; Actin related protein 2/3 complex subunit 5 antibody; Actin related protein 2/3 complex; subunit 5 16kDa antibody; Actin-related protein 2/3 complex subunit 5 antibody; ARC16 antibody; Arp2/3 complex 16 kDa subunit antibody; Arp2/3 protein complex subunit p16 antibody; ARPC 5 antibody; Arpc5 antibody; ARPC5_HUMAN antibody; dJ127C7.3 antibody; MGC88523 antibody; p16 Arc antibody; p16-ARC antibody; RP1 127C7.3 antibody; anti-ARPC5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SR34-02
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-ARPC5 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC: 1:50
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of p16 ARC on different cell lysates using anti-p16 ARC antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: SK-BR-3)

Western Blot (WB) (Western blot analysis of p16 ARC on different cell lysates using anti-p16 ARC antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: SK-BR-3)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-p16 ARC antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-p16 ARC antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-p16 ARC antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-p16 ARC antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-p16 ARC antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-p16 ARC antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining p16 ARC in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining p16 ARC in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of SH-SY-5Y cells with p16 ARC antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of SH-SY-5Y cells with p16 ARC antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody.)
Related Product Information for anti-ARPC5 antibody
The Arp2/3 (Actin-related protein 2/3) complex consists of seven subunits, all of which are actin-related proteins. The complex is involved in the control of actin polymerization and in mediating the formation of branched actin networks. p16-ARC, also known as ARPC5 (Actin-related protein 2/3 complex subunit 5) or ARC16 (Arp2/3 complex 16 kDa subunit), is a 151 amino acid subunit of the Arp2/3 complex. Thought to play a role in maintaining the integrity of Arp2/3, p16-ARC is a substrate for MAPKAPK-2 which, through phosphorylation of p16-ARC, may participate in Arp2/3 regulatory functions and remodeling of the Actin cytoskeleton. Two isoforms of p16-ARC exist due to alternative splicing events.
Product Categories/Family for anti-ARPC5 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,631 Da
NCBI Official Full Name
actin-related protein 2/3 complex subunit 5 isoform 2
NCBI Official Synonym Full Names
actin related protein 2/3 complex, subunit 5, 16kDa
NCBI Official Symbol
ARPC5
NCBI Official Synonym Symbols
ARC16; p16-Arc; dJ127C7.3
NCBI Protein Information
actin-related protein 2/3 complex subunit 5; Arp2/3 protein complex subunit p16; arp2/3 complex 16 kDa subunit
UniProt Protein Name
Actin-related protein 2/3 complex subunit 5
UniProt Gene Name
ARPC5
UniProt Synonym Gene Names
ARC16; p16-ARC
UniProt Entry Name
ARPC5_HUMAN

NCBI Description

This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p16 subunit, has yet to be determined. Alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

ARPC5: Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Belongs to the ARPC5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 1q25.3

Cellular Component: Arp2/3 protein complex; focal adhesion; cell projection; cytoplasm; cytosol; actin cytoskeleton

Molecular Function: actin filament binding; protein binding; structural constituent of cytoskeleton

Biological Process: axon guidance; ephrin receptor signaling pathway; innate immune response; actin cytoskeleton organization and biogenesis; cell motility

Research Articles on ARPC5

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Product Notes

The ARPC5 arpc5 (Catalog #AAA9435514) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p16 ARC Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's p16 ARC can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:50 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the ARPC5 arpc5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p16 ARC, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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