IL1A active protein

Recombinant Mouse IL1A

Cat. Num. AAA4159926

• Gene Names: Il1a; Il-1a
• Purity: >95% as determined by SDS-PAGE
• Synonyms: IL1A; Recombinant Mouse IL1A; IL-1 alpha; IL1a; Recombinant Mouse Interleukin-1 Alpha (IL1A); IL1A active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered PBS solution.
• Sequence Length: 270

• Bioactivity: The ED(50) was determined by a cell proliferation assay using the murine helper T cell line, D10.G4., was found to be 10.0 pg/mL.
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Testing Data

Related Product Information for IL1A active protein

Monocytes are the main source of secreted IL-1. They express predominantly IL-1-beta while human keratinocytes express large amounts of IL-1-alpha. Murine macrophages display a transition from IL-1-beta to IL-1-alpha production during maturation of monocytes into inflammatory macrophages. There are two functionally almost equivalent forms of IL-1, IL-1-alpha and IL-1-beta that are encoded by two different genes. IL-1-beta is the predominant form in humans while it is IL-1-alpha in mice. Both forms of IL-1 bind to the same receptor and therefore also show similar if not identical biological activities. The IL-1-beta but not the IL-1-alpha precursor must be processed before it can bind to the receptor. Both forms of IL-1 bind to the same receptor and therefore also show similar if not identical biological activities. The receptor isolated from T-cells is expressed predominantly on T cells and cells of mesenchymal origin. It binds both types of IL-1 with equal affinity. This type is called also Type 1 receptor. It has been designated CD121a. The Type 2 receptor has been designated CD121b. It is isolated from B-cells, granulocytes, and macrophages. It is expressed predominantly on B-cells and cells of the myelomonocytic lineage and is encoded by a separate gene.

NCBI and Uniprot Product Information

• NCBI GI #: 47059075
• NCBI GeneID: 16175
• NCBI Accession #: NP_034684.2
• NCBI GenBank Nucleotide #: NP_034684.2
• UniProt Accession #: P01582
• Molecular Weight: 18.0 kDa
• NCBI Official Full Name: interleukin-1 alpha
• NCBI Official Synonym Full Names: interleukin 1 alpha
• NCBI Official Symbol: Il1a
• NCBI Official Synonym Symbols: Il-1a
• NCBI Protein Information: interleukin-1 alpha
• UniProt Protein Name: Interleukin-1 alpha
• Protein Family: Interleukin
• UniProt Gene Name: Il1a
• UniProt Synonym Gene Names: IL-1 alpha
• UniProt Entry Name: IL1A_MOUSE

Uniprot Description

IL1A: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Belongs to the IL-1 family.

Protein type: Cytokine; Motility/polarity/chemotaxis

Cellular Component: cell surface; cytoplasm; cytosol; extracellular space; plasma membrane

Molecular Function: copper ion binding; cytokine activity; interleukin-1 receptor binding

Biological Process: connective tissue replacement during inflammatory response; cytokine and chemokine mediated signaling pathway; germ cell programmed cell death; inflammatory response; keratinization; negative regulation of cell proliferation; positive regulation of angiogenesis; positive regulation of cytokine secretion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-6 production; positive regulation of JNK cascade; positive regulation of mitosis; positive regulation of prostaglandin secretion; positive regulation of protein secretion; positive regulation of stress-activated MAPK cascade; positive regulation of transcription from RNA polymerase II promoter; regulation of actin cytoskeleton organization and biogenesis; regulation of sensory perception of pain; response to copper ion