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Western Blot (WB) (Western Blot detection against Immunogen (89.21kD).)

Mouse anti-Human ORC2 Monoclonal Antibody | anti-ORC2 antibody

ORC2 (ORC2L, Origin Recognition Complex, Subunit 2-Like (yeast), Orc2, Origin Of Replication 2 (Saccharomyces Homolog)-Like, Origin Recognition Complex, Subunit 2 (yeast homolog)-Like) (Biotin)

Gene Names
ORC2; ORC2L
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ORC2; Monoclonal Antibody; ORC2 (ORC2L; Origin Recognition Complex; Subunit 2-Like (yeast); Orc2; Origin Of Replication 2 (Saccharomyces Homolog)-Like; Subunit 2 (yeast homolog)-Like) (Biotin); anti-ORC2 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3E11-1G5
Specificity
Recognizes human ORC2L.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Sequence Length
2815
Applicable Applications for anti-ORC2 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 5ug/ml
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-578 from human ORC2L (AAH14834) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MSKPELKEDKMLEVHFVGDDDVLNHILDREGGAKLKKERAQLLVNPKKIIKKPEYDLEEDDQEVLKDQNYVEIMGRDVQESLKNGSATGGGNKVYSFQNRKHSEKMAKLASELAKTPQKSVSFSLKNDPEITINVPQSSKGHSASDKVQPKNNDKSEFLSTAPRSLRKRLIVPRSHSDSESEYSASNSEDDEGVAQEHEEDTNAVIFSQKIQAQNRVVSAPVGKETPSKRMKRDKTSDLVEEYFEAHSSSKVLTSDRTLQKLKRAKLDQQTLRNLLSKVSPSFSAELKQLNQQYEKLFHKWMLQLHLGFNIVLYGLGSKRDLLERFRTTMLQDSIHVVINGFFPGISVKSVLNSITEEVLDHMGTFRSILDQLDWIVNKFKEDSSLELFLLIHNLDSQMLRGEKSQQIIGQLSSLHNIYLIASIDHLNAPLMWDHAKQSLFNWLWYETTTYSPYTEETSYENSLLVKQSGSLPLSSLTHVLRSLTPNARGIFRLLIKYQLDNQDNPSYIGLSFQDFYQQCREAFLVNSDLTLRAQLTEFRDHKLIRTKKGTDGVEYLLIPVDNGTLTDFLEKEEEEA
Conjugate
Biotin
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (89.21kD).)

Western Blot (WB) (Western Blot detection against Immunogen (89.21kD).)

Western Blot (WB)

(ORC2L monoclonal antibody Western Blot analysis of ORC2L expression in Hela NE.)

Western Blot (WB) (ORC2L monoclonal antibody Western Blot analysis of ORC2L expression in Hela NE.)

Western Blot (WB)

(ORC2L monoclonal antibody Western Blot analysis of ORC2L expression in Jurkat.)

Western Blot (WB) (ORC2L monoclonal antibody Western Blot analysis of ORC2L expression in Jurkat.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to ORC2L on formalin-fixed paraffin-embedded human spleen tissue.[antibody concentration 5ug/ml])

Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to ORC2L on formalin-fixed paraffin-embedded human spleen tissue.[antibody concentration 5ug/ml])

Testing Data

(Detection limit for recombinant GST tagged ORC2L is ~0.3ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged ORC2L is ~0.3ng/ml as a capture antibody.)
Product Categories/Family for anti-ORC2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Official Full Name
Homo sapiens origin recognition complex, subunit 2-like (yeast), mRNA
NCBI Official Synonym Full Names
origin recognition complex subunit 2
NCBI Official Symbol
ORC2
NCBI Official Synonym Symbols
ORC2L
NCBI Protein Information
origin recognition complex subunit 2

NCBI Description

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3, -4, and -5. It also interacts with CDC45 and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication. Alternatively spliced transcript variants have been found, one of which is a nonsense-mediated mRNA decay candidate. [provided by RefSeq, Oct 2010]

Research Articles on ORC2

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Product Notes

The ORC2 (Catalog #AAA6143319) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ORC2 (ORC2L, Origin Recognition Complex, Subunit 2-Like (yeast), Orc2, Origin Of Replication 2 (Saccharomyces Homolog)-Like, Origin Recognition Complex, Subunit 2 (yeast homolog)-Like) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ORC2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 5ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ORC2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ORC2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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