anti-Human NUP98 Monoclonal Antibody | anti-NUP98 antibody

Anti-NUP98 Mouse mAb

Cat. Num. AAA475002

• Gene Names: NUP98; ADIR2; NUP96; NUP196
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified
• Synonyms: NUP98; Monoclonal Antibody; Anti-NUP98 Mouse mAb; 96 kDa nucleoporin; 98 kDa nucleoporin; ADAR2; ADIR2; GLFG-repeat containing nucleoporin; Nuclear pore complex protein Nup96; Nuclear pore complex protein Nup98 Nup96; Nucleoporin 98kD; nucleoporin 98kDa; Nucleoporin Nup96; Nucleoporin Nup98; NUP196; NUP96; Nup98; Nup98-Nup96; NUP98_HUMAN; anti-NUP98 antibody

• Host: Mouse; Source: Human
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3B8-D7-H10
• Purity/Purification: Affinity Purified
• Form/Format: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 937

• Applicable Applications for anti-NUP98 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000
• Immunogen: Purified recombinant human NUP98 protein fragments expressed in E Coli.
• Sensitivity: This antibody detects endogenous levels of NUP98 and does not cross-react with related proteins.
• Sequence Similarities: Belongs to the nucleoporin GLFG family. Contains 1 peptidase S59 domain.
• Subunit Structure: Nup98 interacts directly with Nup96. Nup96 is part of the Nup160 subcomplex in the nuclear pore which is composed of Nup160, Nup133, Nup107 and Nup96. This complex plays a role in RNA export and in tethering Nup98 and Nup153 to the nucleus. May interact with RAE1. Interacts with vesicular stomatitis virus protein M.
• Subcellular Location: Nucleus; nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side.

Western Blot (WB)

(Western blot detection of NUP98 in Hela, Jurkat and MCF7 cell lysates using NUP98 mouse mAb (1:1000 diluted).Predicted band size: 98kDa.Observed band size: 98kDa.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of Hela cell lysates using NUP98 mouse mAb.)

Related Product Information for anti-NUP98 antibody

Entrez Summary: Signal-mediated nuclear import and export proceed through the nuclear pore complex (NPC), which is comprised of approximately 50 unique proteins collectively known as nucleoporins. The 98 kDa nucleoporin is generated through a biogenesis pathway that involves synthesis and proteolytic cleavage of a 186 kDa precursor protein. This cleavage results in the 98 kDa nucleoporin as well as a 96 kDa nucleoporin, both of which are localized to the nucleoplasmic side of the NPC. Rat studies show that the 98 kDa nucleoporin functions as one of several docking site nucleoporins of transport substrates. The human gene has been shown to fuse to several genes following chromosome translocations in acute myelogenous leukemia (AML) and T-cell acute lymphocytic leukemia (T-ALL). This gene is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described.
UniPort Summary Function: Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport.

Product Categories/Family for anti-NUP98 antibody

Tags & Cell Markers; Subcellular Markers; Nucleus; Nuclear Pore

NCBI and Uniprot Product Information

• NCBI GI #: 56549643
• NCBI GeneID: 4928
• NCBI Accession #: NP_005378.4
• NCBI GenBank Nucleotide #: NM_016320.3
• UniProt Accession #: P52948; Q8IUT2; Q8WYB0; Q96E54; Q9H3Q4; Q9NT02; Q9UF57; Q9UHX0; Q9Y6J4; Q9Y6J5
• Molecular Weight: 98
• NCBI Official Full Name: nuclear pore complex protein Nup98 isoform 3 proprotein
• NCBI Official Synonym Full Names: nucleoporin 98
• NCBI Official Symbol: NUP98
• NCBI Official Synonym Symbols: ADIR2; NUP96; NUP196
• NCBI Protein Information: nuclear pore complex protein Nup98-Nup96; nuclear pore complex protein Nup98
• UniProt Protein Name: Nuclear pore complex protein Nup98-Nup96
• UniProt Gene Name: NUP98
• UniProt Synonym Gene Names: ADAR2; Nup98; Nup96

NCBI Description

Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and encodes a 186 kDa precursor protein that undergoes autoproteolytic cleavage to generate a 98 kDa nucleoporin and 96 kDa nucleoporin. The 98 kDa nucleoporin contains a Gly-Leu-Phe-Gly (GLGF) repeat domain and participates in many cellular processes, including nuclear import, nuclear export, mitotic progression, and regulation of gene expression. The 96 kDa nucleoporin is a scaffold component of the NPC. Proteolytic cleavage is important for targeting of the proteins to the NPC. Translocations between this gene and many other partner genes have been observed in different leukemias. Rearrangements typically result in chimeras with the N-terminal GLGF domain of this gene to the C-terminus of the partner gene. Alternative splicing results in multiple transcript variants encoding different isoforms, at least two of which are proteolytically processed. Some variants lack the region that encodes the 96 kDa nucleoporin. [provided by RefSeq, Feb 2016]

Uniprot Description

Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Nup98 and Nup96 are involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC.