NUP98 Polyclonal Antibody | anti-NUP98 antibody

Anti-NUP98 Antibody

Cat. Num. AAA178198

• Gene Names: NUP98; ADIR2; NUP96; NUP196
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: NUP98; Polyclonal Antibody; Anti-NUP98 Antibody; Nuclear pore complex protein Nup98-Nup96; 96 kDa nucleoporin; 98 kDa nucleoporin; ADAR2; ADIR2; GLFG-repeat containing nucleoporin; Nuclear pore complex protein Nup96; Nuclear pore complex protein Nup98 Nup96; Nucleoporin 98kD; nucleoporin 98kDa; Nucleoporin Nup96; Nucleoporin Nup98; NUP196; NUP96; Nup98; Nup98-Nup96; NUP98_HUMAN; anti-NUP98 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 937

• Applicable Applications for anti-NUP98 antibody: Western Blot (WB)
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml
• Immunogen: E Coli-derived human NUP98 recombinant protein (Position: H549-F880). Human NUP98 shares 95% and 94% amino acid (aa) sequence identity with mouse and rat NUP98, respectively.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- NUP98 Picoband antibody, MBS178198, Western blottingAll lanes: Anti NUP98 (MBS178198) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Mouse Liver Tissue Lysate at 50ugLane 3: Mouse Lung Tissue Lysate at 50ugLane 4: A549 Whole Cell Lysate at 40ugLane 5: SMMC Whole Cell Lysate at 40ugPredicted bind size: 98KDObserved bind size: 98KD )

Related Product Information for anti-NUP98 antibody

Description: Rabbit IgG polyclonal antibody for Nuclear pore complex protein Nup98-Nup96(NUP98) detection. Tested with WB in Human;Mouse;Rat.

Background: Nuclear pore complex protein Nup98-Nup96 is a protein that in humans is encoded by the NUP98 gene. This gene is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. NUP98 is a peripheral nucleoporin located at both the cytoplasmic and nuclear sides of the central channel of the NPC. NUP98 phosphorylation is critical for NPC disassembly at the onset of mitosis. It also plays roles in gene expression, mitotic checkpoint, and pathogenesis. Ligand blot analysis suggested that NUP98 can function as a docking protein for cytosol-mediated docking of import substrates. In addition to that, NUP98 is a target of the vesicular stomatitis virus M protein-mediated inhibition of mRNA nuclear export.

References

1. Laurell, E., Beck, K., Krupina, K., Theerthagiri, G., Bodenmiller, B., Horvath, P., Aebersold, R., Antonin, W., Kutay, U. Phosphorylation of Nup98 by multiple kinases is crucial for NPC disassembly during mitotic entry. Cell 144: 539-550, 2011. 2. Nakamura, T., Largaespada, D. A., Lee, M. P., Johnson, L. A., Ohyashiki, K., Toyama, K., Chen, S. J., Willman, C. L., Chen, I.-M., Feinberg, A. P., Jenkins, N. A., Copeland, N. G., Shaughnessy, J. D., Jr. Fusion of the nucleoporin gene NUP98 to HOXA9 by the chromosome translocation t(7;11)(p15;p15) in human myeloid leukaemia. Nature Genet. 12: 154-158, 1996. 3. von Kobbe, C., van Deursen, J. M. A., Rodrigues, J. P., Sitterlin, D., Bachi, A., Wu, X., Wilm, M., Carmo-Fonseca, M., Izaurralde, E. Vesicular stomatitis virus matrix protein inhibits host cell gene expression by targeting the nucleoporin Nup98. Molec. Cell 6: 1243-1252, 2000.

NCBI and Uniprot Product Information

• NCBI GI #: 56549643
• NCBI GeneID: 4928
• NCBI Accession #: NP_005378.4
• NCBI GenBank Nucleotide #: NM_005387.6
• UniProt Accession #: P52948; Q8IUT2; Q8WYB0; Q96E54; Q9H3Q4; Q9NT02; Q9UF57; Q9UHX0; Q9Y6J4; Q9Y6J5
• Molecular Weight: 186,268 Da
• NCBI Official Full Name: nuclear pore complex protein Nup98 isoform 3 proprotein
• NCBI Official Synonym Full Names: nucleoporin 98kDa
• NCBI Official Symbol: NUP98
• NCBI Official Synonym Symbols: ADIR2; NUP96; NUP196
• NCBI Protein Information: nuclear pore complex protein Nup98; nuclear pore complex protein Nup98-Nup96
• UniProt Protein Name: Nuclear pore complex protein Nup98-Nup96
• UniProt Gene Name: NUP98
• UniProt Synonym Gene Names: ADAR2; Nup98; Nup96
• UniProt Entry Name: NUP98_HUMAN

NCBI Description

Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and encodes a 186 kDa precursor protein that undergoes autoproteolytic cleavage to generate a 98 kDa nucleoporin and 96 kDa nucleoporin. The 98 kDa nucleoporin contains a Gly-Leu-Phe-Gly (GLGF) repeat domain and participates in many cellular processes, including nuclear import, nuclear export, mitotic progression, and regulation of gene expression. The 96 kDa nucleoporin is a scaffold component of the NPC. Proteolytic cleavage is important for targeting of the proteins to the NPC. Translocations between this gene and many other partner genes have been observed in different leukemias. Rearrangements typically result in chimeras with the N-terminal GLGF domain of this gene to the C-terminus of the partner gene. Alternative splicing results in multiple transcript variants encoding different isoforms, at least two of which are proteolytically processed. Some variants lack the region that encodes the 96 kDa nucleoporin. [provided by RefSeq, Feb 2016]

Uniprot Description

Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Nup98 and Nup96 are involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC.