Mouse NR2B Monoclonal Antibody | anti-Grin2b antibody

NR2B Antibody: ATTO 390

Cat. Num. AAA802089

• Gene Names: Grin2b; GluN2B
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Synonyms: NR2B; Monoclonal Antibody; NR2B Antibody: ATTO 390; NMDAR2B; hNR3; MGC142180; glutamate receptor subunit epsilon-2; NR3; anti-Grin2b antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: S59-36
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 1482

• Applicable Applications for anti-Grin2b antibody: Western (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)
• Conjugate: ATTO 390
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunocytochemistry/Immunofluorescence (ICC/IF)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything positive.)

Western Blot (WB)

(Western Blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:250.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining, and dermal staining.)

Western Blot (WB)

(Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Related Product Information for anti-Grin2b antibody

Background Info: Detects ~166kDa. No cross-reactivity against NR2A

Scientific Background: NR2B containing receptors have been implicated in synaptic plasticity, memory formation and pain modulation (3). Studies suggest that the NR2B subunit of glutamate receptors may be potential targets for relieving pain; NR2B may be a probable target for anti-nociceptive drugs, and may also be useful as analgesics (4).

Product Categories/Family for anti-Grin2b antibody

Ion Channels; Neuroscience

References

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Zhuo M. (2009) Mol Brain. 2(1): 4 4. Gurwitz D, and Weizman A. (2002) Drug Discovery Today, 7(7): 403-406.

NCBI and Uniprot Product Information

• NCBI GI #: 6980984
• NCBI GeneID: 24410
• NCBI Accession #: NP_036706.1
• NCBI GenBank Nucleotide #: NM_012574.1
• UniProt Accession #: Q00960; Q62684
• Molecular Weight: 166,071 Da
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 2B
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• NCBI Official Symbol: Grin2b
• NCBI Official Synonym Symbols: GluN2B
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 2B; NR2B; NMDAR2B; glutamate receptor, ionotropic, NMDA2B; N-methyl D-aspartate receptor subtype 2B; glutamate [NMDA] receptor subunit epsilon-2
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 2B
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: Grin2b
• UniProt Synonym Gene Names: GluN2B; NMDAR2B; NR2B
• UniProt Entry Name: NMDE2_RAT

NCBI Description

subunit of the NMDA receptor glutamate gated ion channel; displays voltage dependent sensitivity to magnesium, calcium permeability, and glycine regulated activity; may facilitate learning and long term potentiation [RGD, Feb 2006]

Uniprot Description

NMDAR2B: an NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Channel, ligand-gated; Channel, calcium

Cellular Component: presynaptic membrane; postsynaptic membrane; synaptic vesicle; neuron projection; cell surface; membrane; postsynaptic density; dendritic spine; terminal button; synapse; cell junction; Z disc; N-methyl-D-aspartate selective glutamate receptor complex

Molecular Function: neurotransmitter binding; interleukin-1 receptor binding; zinc ion binding; calcium channel activity; beta-catenin binding; drug binding; ionotropic glutamate receptor binding; protein binding; extracellular-glutamate-gated ion channel activity; ionotropic glutamate receptor activity; protein heterodimerization activity; glycine binding; N-methyl-D-aspartate selective glutamate receptor activity; cation channel activity; cell adhesion molecule binding; D2 dopamine receptor binding; receptor binding

Biological Process: regulation of long-term neuronal synaptic plasticity; fear response; startle response; behavioral fear response; response to toxin; rhythmic process; positive regulation of glutamate secretion; regulation of synaptic plasticity; response to other organism; receptor clustering; response to organic cyclic substance; positive regulation of synaptic transmission; response to carbohydrate stimulus; synaptic transmission; response to magnesium ion; behavioral response to pain; learning and/or memory; calcium ion transport; response to methylmercury; response to electrical stimulus; associative learning; cation transport; cellular response to magnesium starvation; synaptic transmission, glutamatergic; regulation of action potential; in utero embryonic development; response to amphetamine; hippocampus development; learning; response to cocaine; memory; response to manganese ion; detection of mechanical stimulus involved in sensory perception of pain; regulation of postsynaptic membrane potential; response to ethanol; sensory organ development; long-term memory; suckling behavior; ionotropic glutamate receptor signaling pathway; response to mechanical stimulus; regulation of MAPKKK cascade; response to cytokine stimulus; cerebral cortex development; response to calcium ion; regulation of excitatory postsynaptic membrane potential; multicellular organismal response to stress; response to amine stimulus