Mouse Nav1.8 Monoclonal Antibody | anti-Scn10a antibody

Nav1.8 Antibody: RPE

Cat. Num. AAA800796

• Gene Names: Scn10a; PN3; Na(V)1.8
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Synonyms: Nav1.8; Monoclonal Antibody; Nav1.8 Antibody: RPE; PN3; Na(V)1.8; Scn10a; anti-Scn10a antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: S134-12
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 1957

• Applicable Applications for anti-Scn10a antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)
• Conjugate: RPE
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunocytochemistry/Immunofluorescence (ICC/IF)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134-12. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic staining and some dull nuclear staining.)

Western Blot (WB)

(Western Blot analysis of Monkey COS transient cell lysate showing detection of Nav1.8 protein using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134-12. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134-12. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Heavy filaggrin-like staining, lower epidermal cells have some staining.)

Related Product Information for anti-Scn10a antibody

Background Info: Detects ~220kDa. No cross reactivity against other Nav channels

Scientific Background: Nav1.8 is a voltage-gated sodium channel and plays a critical role in the generation and conduction of action potentials and is thus important for electrical signaling by most excitable cells. Therapeutically, the association of pain insensitivity with the loss of function of a certain sodium channel may have implications. Since Nav1.8 is not present in cardiac muscle or neurons in the central nervous system, blockers of Nav1.8 will not have direct action on these cells and thus can have less side effects than current pain medications. By performing more studies, there is a possibility to develop a new generation of drugs that can reduce the pain intensity in animals (3).

Product Categories/Family for anti-Scn10a antibody

Ion Channels; Neuroscience

References

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Dray A. (2008) Br. J. Anaesth. 101(1): 48-58. 4. Dray A., Read S.J (2007) Arthritis Res. Ther. 9(3): 212. 5. Samuels M.E., teMorshe R.H., Lynch M.E., Drenth J.P. (2008) Mol Pain. 4: 21.

NCBI and Uniprot Product Information

• NCBI GI #: 8394242
• NCBI GeneID: 29571
• NCBI Accession #: NP_058943.1
• NCBI GenBank Nucleotide #: NM_017247.1
• UniProt Accession #: Q62968; Q63554; Q6EWG6
• Molecular Weight: 219,605 Da
• NCBI Official Full Name: sodium channel protein type 10 subunit alpha
• NCBI Official Synonym Full Names: sodium channel, voltage-gated, type X, alpha subunit
• NCBI Official Symbol: Scn10a
• NCBI Official Synonym Symbols: PN3; Na(V)1.8
• NCBI Protein Information: sodium channel protein type 10 subunit alpha; sensory neuron sodium channel; peripheral nerve sodium channel 3; sodium channel type X alpha polypeptide; sodium channel protein type X subunit alpha; voltage-gated sodium channel subunit alpha Nav1.8; sodium channel voltage-gated type X alpha polypeptide; sodium channel, voltage-gated, type 10, alpha polypeptide
• UniProt Protein Name: Sodium channel protein type 10 subunit alpha
• Protein Family: Sodium channel protein
• UniProt Gene Name: Scn10a
• UniProt Synonym Gene Names: Sns; PN3
• UniProt Entry Name: SCNAA_RAT

NCBI Description

voltage-gated sodium channel that is resistant to tetrodotoxin [RGD, Feb 2006]

Uniprot Description

SCN10A: This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant sodium channel isoform. Its electrophysiological properties vary depending on the type of the associated beta subunits (in vitro). Plays a role in neuropathic pain mechanisms. Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, sodium