Testing Data
Rabbit anti-Human Nonstructural Protein 8 (NSP8) Polyclonal Antibody | anti-NSP8 antibody
Anti-SARS-CoV Nonstructural Protein 8 (NSP8) Antibody
Reactivity
Human
Applications
Western Blot
Purity
Purified
Synonyms
Nonstructural Protein 8 (NSP8); Polyclonal Antibody; Anti-SARS-CoV Nonstructural Protein 8 (NSP8) Antibody; SARS-CoV(N) Protein; anti-NSP8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody is directed against SARS-Coronavirus nsp8 protein. The product is neat antiserum. Cross reactivity with homologues from other sources has not been determined.
Purity/Purification
Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Sequence Length
523
Applicable Applications for anti-NSP8 antibody
Western Blot (WB)
Application Notes
This antibody has been tested for use in immunofluorescence microscopy, immunoelectron microscopy, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 22 kDa in size corresponding to SARS-CoV nsp8 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37 degree C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.
Immunogen
This whole rabbit serum was produced by repeated immunizations with a purified His-tagged recombinant protein corresponding to full-length SARS-Coronavirus nsp8.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-NSP8 antibody
The nonstructural protein 8 (nsp8) is one of the SARS-Coronavirus replicase cleaving products_recover, encoded by ORF1a. Nsp8 is thought to be part of the viral replication complex, which is associated with intracellular membranes. No specific information on the function of nsp8 is available.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
nonstructural protein NSP8, partial
Similar Products
Product Notes
The NSP8 (Catalog #AAA4157746) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SARS-CoV Nonstructural Protein 8 (NSP8) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nonstructural Protein 8 (NSP8) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). This antibody has been tested for use in immunofluorescence microscopy, immunoelectron microscopy, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 22 kDa in size corresponding to SARS-CoV nsp8 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37 degree C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min. Researchers should empirically determine the suitability of the NSP8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nonstructural Protein 8 (NSP8), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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