Mouse NKX2.2 Monoclonal Antibody | anti-NKX2.2 antibody

NKX2.2 (Homeobox Protein NK-2 Homolog B, NK2 Transcription factor like Protein B, NK2 Transcription factor related locus 2, NKX22, Nkx2b, tinman) (HRP)

Cat. Num. AAA6124412

• Gene Names: NKX2-2; NKX2B; NKX2.2
• Reactivity: Chicken, Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: NKX2.2; Monoclonal Antibody; NKX2.2 (Homeobox Protein NK-2 Homolog B; NK2 Transcription factor like Protein B; NK2 Transcription factor related locus 2; NKX22; Nkx2b; tinman) (HRP); Homeobox Protein NK-2 Homolog B; tinman; anti-NKX2.2 antibody

• Host: Mouse
• Reactivity: Chicken, Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: SPM564
• Specificity: Recognizes NKX2.2. Species Crossreactivity: Human, Mouse, Rat and Chicken.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-NKX2.2 antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IP: 1-2ug/500ug protein lysate; WB: 0.5-1ug/ml; IHC: 0.5-1ug/ml for 30 minutes at RT; Applications are based on unconjugated antibody.
• Immunogen: Human NKX2.2 recombinant protein. Cellular Localization: Nuclear
• Conjugate: HRP
• Positive Control: Pancreas or Ewing sarcoma
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-paraffin Ewing's sarcoma stained with NKX2.2 MAb (SPM564).)

Immunohistochemistry (IHC)

(Formalin-paraffin pancreas stained with NKX2.2 MAb (SPM564).)

Product Categories/Family for anti-NKX2.2 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

1. Wang, Y.C. et al. (2009) Endocr Relat Cancer 16, 267-79. 2. Yoshida A et. al. Am J Surg Pathol. 2012;36(7):993-9

NCBI and Uniprot Product Information

• NCBI GI #: 4505401
• NCBI GeneID: 4821
• NCBI Accession #: NP_002500
• NCBI GenBank Nucleotide #: NM_002509.3
• UniProt Accession #: O95096
• Molecular Weight: 30,133 Da
• NCBI Official Full Name: homeobox protein Nkx-2.2
• NCBI Official Synonym Full Names: NK2 homeobox 2
• NCBI Official Symbol: NKX2-2
• NCBI Official Synonym Symbols: NKX2B; NKX2.2
• NCBI Protein Information: homeobox protein Nkx-2.2; NK-2 homolog B; homeobox protein NK-2 homolog B; NK2 transcription factor-like protein B; NK2 transcription factor related, locus 2
• UniProt Protein Name: Homeobox protein Nkx-2.2
• UniProt Gene Name: NKX2-2
• UniProt Synonym Gene Names: NKX2.2; NKX2B
• UniProt Entry Name: NKX22_HUMAN

NCBI Description

The protein encoded by this gene contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor. [provided by RefSeq, Jul 2008]

Uniprot Description

NKX2-2: Acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter. Belongs to the NK-2 homeobox family.

Protein type: Cell development/differentiation; Transcription factor

Chromosomal Location of Human Ortholog: 20p11.22

Cellular Component: nucleoplasm

Molecular Function: sequence-specific DNA binding; transcription coactivator activity; chromatin binding; transcription factor activity; transcription factor binding

Biological Process: smoothened signaling pathway; transcription, DNA-dependent; spinal cord oligodendrocyte cell fate specification; spinal cord motor neuron differentiation; endocrine pancreas development; negative regulation of neuron differentiation; astrocyte differentiation; gut development; optic nerve development; response to glucose stimulus; neuron fate specification; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; brain development; positive regulation of oligodendrocyte differentiation; oligodendrocyte development; positive regulation of neuron differentiation; response to progesterone stimulus