Rabbit anti-Human UCK1 Polyclonal Antibody | anti-UCK1 antibody

Anti-UCK1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8109187

• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: UCK1; Polyclonal Antibody; Anti-UCK1 Antibody; Rabbit Polyclonal; UCK1 Antibody; Rabbit PAb; Antigen Affinity Purified; uridine-cytidine kinase 1; Anti-URK1 Antibody; anti-UCK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human UCK1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-UCK1 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:200-1:1000; ICC/IF: 1:100-1:500
• Immunogen: E Coli-derived Human UCK1 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human UCK1 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human UCK1 in human liver with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human UCK1 in human large intestine with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of UCK1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human UCK1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)

Related Product Information for anti-UCK1 antibody

The uridine/cytidine kinase (UCK) family consists of two members, UCK1 and UCK2, and both UCKs are expressed in many tumor cells. UCK1 has an established role in activating 5'-azacytidine (5'-AZA) and its protein level is significantly downregulated in patients resistant to the drug. The KLHL2/USP28/ATM axis mediates resistance of AML cells to 5'-AZA by regulating UCK1 ubiquitination and phosphorylation. UCK2, an enzyme normally expressed in human placenta and testis and highly overexpressed in many neoplasias of blood and solid tissues, catalyzes monophosphorylation of pyrimidine ribonucleosides with efficiency 15-to 20-fold higher than that of ubiquitously expressed isozyme UCK1. Uridine-cytidine kinases (encoded by UCK1, UCKL1, and UCK2) catalyze the phosphorylation of uridine and cytidine to uridine monophosphate (UMP) and cytidine monophosphate (CMP).

References

Zlatopolskiy BD, et al. (2009) Synthesis and biologic study of iv-14, a new ribonucleoside radiotracer for tumor visualization. J Nucl Med 50 (11): 1895-1903.Zhang H, et al. (2020) Deubiquitinase usp28 inhibits ubiquitin ligase klhl2-mediated uridine-cytidine kinase 1 degradation and confers sensitivity to 5'-azacytidine-resistant human leukemia cells. Theranostics 10 (3): 1046-1059.Yu S, et al. (2019) Uck2 upregulation might serve as an indicator of unfavorable prognosis of hepatocellular carcinoma. IUBMB Life 71 (1): 105-112.Murata D, et al. (2004) A crucial role of uridine/cytidine kinase 2 in antitumor activity of 3'-ethynyl nucleosides. Drug Metab Dispos 32 (10): 1178-1182.