Mouse anti-Human NHERF1 Monoclonal Antibody | anti-SLC9A3R1 antibody

NHERF1 (Na+/H+ Exchanger Regulatory Factor, Na(+)/H(+) Exchange Regulatory Cofactor NHE-RF1, NHERF-1, NHERF, Ezrin-Radixin-Moesin Binding Phosphoprotein 50, EBP50, NPHLOP2, Regulatory Cofactor of Na(+)/H(+) Exchanger, Sodium-hydrogen Exchanger Regulatory

Cat. Num. AAA631837

• Gene Names: SLC9A3R1; EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: NHERF1; Monoclonal Antibody; NHERF1 (Na+/H+ Exchanger Regulatory Factor; Na(+)/H(+) Exchange Regulatory Cofactor NHE-RF1; NHERF-1; NHERF; Ezrin-Radixin-Moesin Binding Phosphoprotein 50; EBP50; NPHLOP2; Regulatory Cofactor of Na(+)/H(+) Exchanger; Sodium-hydrogen Exchanger Regulatory; Anti -NHERF1 (Na+/H+ Exchanger Regulatory Factor; anti-SLC9A3R1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: EBP-10
• Specificity: Recognizes human NHERF1.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

• Applicable Applications for anti-SLC9A3R1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Immunoprecipitation and Western Blott.
• Immunogen: Bacterially produced recombinant full-length human NHERF.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-SLC9A3R1 antibody

EBP 50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50kD] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP 50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes.

EBP 50 has been shown to link integral membrane proteins to the cytoskeleton by binding to members of the ERM family of proteins, which bind to F-actin. Through these interactions, EBP 50 is implicated in the localization of interactive groups of proteins into subcellular domains and in the regulation of activity of those interacting proteins. Immunohistochemical studies have shown that EBP 50 is found exclusively at the apical membranes of epithelial cells.

Product Categories/Family for anti-SLC9A3R1 antibody

Antibodies; Abs to Ion Transport

NCBI and Uniprot Product Information

• NCBI GI #: 4759140
• NCBI GeneID: 9368
• NCBI Accession #: NP_004243.1
• NCBI GenBank Nucleotide #: NM_004252.4
• UniProt Accession #: O14745; O43552; Q86WQ5
• Molecular Weight: 38,868 Da
• NCBI Official Full Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• NCBI Official Synonym Full Names: solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
• NCBI Official Symbol: SLC9A3R1
• NCBI Official Synonym Symbols: EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
• NCBI Protein Information: Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; regulatory cofactor of Na(+)/H(+) exchanger; ezrin-radixin-moesin binding phosphoprotein-50; ezrin-radixin-moesin-binding phosphoprotein 50; solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1
• UniProt Protein Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• Protein Family: Na(+)/H(+) exchange regulatory cofactor
• UniProt Gene Name: SLC9A3R1
• UniProt Synonym Gene Names: NHERF; NHERF1; NHERF-1; EBP50
• UniProt Entry Name: NHRF1_HUMAN

NCBI Description

This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer.[provided by RefSeq, Sep 2009]

Uniprot Description

NHERF: a scaffold protein that connects plasma membrane proteins with members of the Ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized beta-2-adrenergic receptor Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: centrosome; microvillus; intracellular membrane-bound organelle; brush border membrane; endomembrane system; lipid raft; actin cytoskeleton; ruffle; membrane; microvillus membrane; apical plasma membrane; cytoplasm; plasma membrane; nucleus; vesicle; filopodium

Molecular Function: protein binding; chloride channel regulator activity; protein self-association; beta-2 adrenergic receptor binding; protein complex binding; beta-catenin binding; dopamine receptor binding; protein complex scaffold; PDZ domain binding; phosphatase binding; receptor binding

Biological Process: bile acid secretion; negative regulation of protein kinase B signaling cascade; Wnt receptor signaling pathway; renal sodium ion transport; negative regulation of cell proliferation; microvillus biogenesis; cellular phosphate ion homeostasis; regulation of sodium:hydrogen antiporter activity; protein complex assembly; dopamine receptor, phospholipase C activating pathway; actin cytoskeleton organization and biogenesis; negative regulation of sodium:hydrogen antiporter activity; regulation of protein kinase activity; negative regulation of cell migration; negative regulation of phosphoinositide 3-kinase cascade; dopamine receptor, adenylate cyclase activating pathway

Disease: Nephrolithiasis/osteoporosis, Hypophosphatemic, 2