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Typical Testing Data/Standard Curve (for reference only)

Mouse Gastric Inhibitory Polypeptide (GIP) ELISA Kit | GIP elisa kit

Mouse Gastric Inhibitory Polypeptide (GIP) ELISA Kit

Reactivity
Mouse
Synonyms
Gastric Inhibitory Polypeptide (GIP); Mouse Gastric Inhibitory Polypeptide (GIP) ELISA Kit; Incretin hormone; Glucose Dependent Insulinotropic Peptide; GIP elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Gastric Inhibitory Polypeptide (GIP).
No significant cross-reactivity or interference between Gastric Inhibitory Polypeptide (GIP) and analogues was observed.
Sequence Length
153
Assay Type
Competitive Inhibition
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
Detection Range
61.7-5,000pg/mL
Sensitivity
< 25.2pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Gastric Inhibitory Polypeptide (GIP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Gastric Inhibitory Polypeptide (GIP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GIP elisa kit
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gastric Inhibitory Polypeptide (GIP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gastric Inhibitory Polypeptide (GIP) and unlabeled Gastric Inhibitory Polypeptide (GIP) (Standards or samples) with the pre-coated antibody specific to Gastric Inhibitory Polypeptide (GIP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gastric Inhibitory Polypeptide (GIP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gastric Inhibitory Polypeptide (GIP) in the sample.
Product Categories/Family for GIP elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,108 Da
NCBI Official Full Name
gastric inhibitory polypeptide preproprotein
NCBI Official Synonym Full Names
gastric inhibitory polypeptide
NCBI Official Symbol
GIP
NCBI Protein Information
gastric inhibitory polypeptide
UniProt Protein Name
Gastric inhibitory polypeptide
UniProt Gene Name
GIP
UniProt Synonym Gene Names
GIP
UniProt Entry Name
GIP_HUMAN

NCBI Description

This gene encodes an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion. [provided by RefSeq, Jul 2008]

Uniprot Description

GIP: Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion. Belongs to the glucagon family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21.3-q22

Cellular Component: extracellular space; cell soma; endoplasmic reticulum lumen; extracellular region

Molecular Function: hormone activity

Biological Process: response to drug; response to peptide hormone stimulus; adult locomotory behavior; positive regulation of insulin secretion; female pregnancy; response to amino acid stimulus; sensory perception of pain; signal transduction; response to lipid; response to organic cyclic substance; endocrine pancreas development; memory; response to starvation; response to selenium ion; cellular protein metabolic process; response to glucose stimulus; response to axon injury; regulation of insulin secretion

Research Articles on GIP

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Product Notes

The Mouse GIP gip (Catalog #AAA2022615) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2022615 ELISA Kit recognizes Mouse GIP. It is sometimes possible for the material contained within the vial of "Gastric Inhibitory Polypeptide (GIP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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