FGF8 active protein

Recombinant Mouse FGF8

Cat. Num. AAA4159975

• Gene Names: FGF8; HH6; AIGF; KAL6; FGF-8; HBGF-8
• Purity: >95% as determined by SDS-PAGE
• Synonyms: FGF8; Recombinant Mouse FGF8; FGF-8; Androgen-induced growth factor; AIGF; Heparin-binding growth factor 8; HBGF-8; Recombinant Mouse Fibroblast Growth Factor 8 (FGF8); FGF8 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered solution in 20mM phosphate buffer, 0.2 mM EDTA and 1M NaCl (pH 8.0)
• Sequence Length: 140

• Bioactivity: The bioactivity was determined in a NIH/3T3 cell proliferation assay. The ED50 was in the range of 0.05-0.5 ng/ml.
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: A quick spin of the vial followed by reconstitution at 100 ug/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Preparation and Storage: Store product at-70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.; For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Testing Data

Testing Data

Related Product Information for FGF8 active protein

FGF-8 (FGF8-b) is a member of the fibroblast growth factor family, which have wide mitogenic and cell survival activities. Additionally, they participate in a number of biological processes during prenatal development, postnatal growth and tissue generation. FGF family members have been implicated in tumour growth, FGF8 specifically in anchorage independent growth of mammary tumour cells. Adult expression of FGF8 is restricted to the testes and ovaries. As an embryonic epithelial growth factor, FGF8 sets up and maintains the midbrain/hindbrain border, and has further roles in limb development, organogenesis and left-axis determination. Recombinant Murine FGF-8b is a 22.5 kDa heparin binding growth factor consisting of 197 amino acids and shares 100% amino acid identity with human FGF-8b.

NCBI and Uniprot Product Information

• NCBI GI #: 329755303
• NCBI GeneID: 2253
• NCBI GenBank Nucleotide #: NP_001193318.1
• Molecular Weight: 22.5 kDa
• NCBI Official Full Name: fibroblast growth factor 8 isoform G
• NCBI Official Synonym Full Names: fibroblast growth factor 8
• NCBI Official Symbol: FGF8
• NCBI Official Synonym Symbols: HH6; AIGF; KAL6; FGF-8; HBGF-8
• NCBI Protein Information: fibroblast growth factor 8

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The adult expression of this gene is restricted to testes and ovaries. Temporal and spatial pattern of this gene expression suggests its function as an embryonic epithelial factor. Studies of the mouse and chick homologs revealed roles in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination. The alternative splicing of this gene results in four transcript variants. [provided by RefSeq, Jul 2008]