Rabbit anti-Human NFAT2 Monoclonal Antibody | anti-NFAT2 antibody

Anti-NFAT2 Rabbit Monoclonal Antibody

Cat. Num. AAA179276

• Gene Names: NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Western Blot
• Purity: Affinity-chromatography
• Synonyms: NFAT2; Monoclonal Antibody; Anti-NFAT2 Rabbit Monoclonal Antibody; Nuclear factor of activated T-cells; cytoplasmic 1; NF-ATc1; NFATc1; NFAT transcription complex cytosolic component; NF-ATc; NFATc; NFATC1; NFATC; anti-NFAT2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Purity/Purification: Affinity-chromatography
• Form/Format: Liquid
• Sequence Length: 353

• Applicable Applications for anti-NFAT2 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: WB: 1:500-1:2000; ICC/IF: 1:50-1:200; FC/FACS: 1:50
• Immunogen: A synthesized peptide derived from human NFAT2
• Subcellular Localization: Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
• Tissue Specificity: Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T- cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T- cells after second antigen exposure. Isoforms IA are widely expressed but not detected in liver nor pancreas, neural expression is strongest in corpus callosum. Isoforms IB are expressed mostly in muscle, cerebellum, placenta and thymus, neural expression in fetal and adult brain, strongest in corpus callosum.
• Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of NFAT2 expression in Ramos cell lysate (MBS179276).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFATC1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NFATC1 )

Related Product Information for anti-NFAT2 antibody

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells.

Product Categories/Family for anti-NFAT2 antibody

Immunology; Adaptive Immunity; T Cells; NON-CD; Signal Transduction; Signaling Pathway; Nuclear Signaling; nfats; Epigenetics and Nuclear Signaling; Transcription; Other Factors; Nuclear Signaling Pathways; Cardiovascular; Heart; Cardiogenesis; Transcript

NCBI and Uniprot Product Information

• NCBI GI #: 522021835
• NCBI GeneID: 4772
• NCBI Accession #: NP_001265598.1
• NCBI GenBank Nucleotide #: NM_001278669.1
• UniProt Accession #: O95644; Q12865; Q15793; Q2M1S3; B5B2M4; B5B2M5; B5B2M6; B5B2M7; B5B2M8; B5B2M9; B5B2N1
• Molecular Weight: 101243 MW
• NCBI Official Full Name: nuclear factor of activated T-cells, cytoplasmic 1 isoform F
• NCBI Official Synonym Full Names: nuclear factor of activated T cells 1
• NCBI Official Symbol: NFATC1
• NCBI Official Synonym Symbols: NFAT2; NFATc; NF-ATC; NF-ATc1.2
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 1
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 1
• UniProt Gene Name: NFATC1
• UniProt Synonym Gene Names: NFAT2; NFATC; NF-ATc1; NFATc1; NF-ATc; NFATc

NCBI Description

The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013]

Uniprot Description

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells (PubMed:10358178). Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function ().