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60 results for " Cardiogenesis" - showing 1-50
Western Blot (WB)
(Figure 1. Western blot analysis of NFAT2 using anti-NFAT2 antibody (MBS1750373).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse thymus tissue lysates,Lane 2: human 22RV1 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NFAT2 at approximately 101KD. The expected band size for NFAT2 is at 101KD. )
NFAT2, Polyclonal Antibody (Cat# AAA1750373)
Full Name
Anti-NFAT2 Picoband Antibody
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat brain lysate showing detection of ~101.2 kDa NFATC1 protein using Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~101.2 kDa. Other Band(s): ~60 kDa isoform.)
NFATC1, Polyclonal Antibody (Cat# AAA8000517)
Full Name
NFATC1 antibody
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~60.4 kDa SMAD4 protein using Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~60.4 kDa.)
SMAD4, Polyclonal Antibody (Cat# AAA8000573)
Full Name
SMAD4 Antibody: ATTO 488
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of MKLP1 expression in HeLa cell lysate (MBS179966).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF23 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KIF23 )
MKLP1, Monoclonal Antibody (Cat# AAA179966)
Full Name
Anti-MKLP1 Rabbit Monoclonal Antibody
Gene Names
KIF23; CHO1; KNSL5; MKLP1; MKLP-1
Reactivity
Human, Mouse, Rat
Applications
IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Islet 1 expression in K562 cell lysate (MBS179920).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ISL1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ISL1 )
Islet 1, Monoclonal Antibody (Cat# AAA179920)
Full Name
Anti-Islet 1 Rabbit Monoclonal Antibody
Gene Names
ISL1; Isl-1; ISLET1
Reactivity
Human, Mouse
Applications
IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Rat brain lysate showing detection of ~101.2 kDa NFATC1 protein using Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~101.2 kDa. Other Band(s): ~60 kDa isoform.)
NFATC1, Polyclonal Antibody (Cat# AAA8000530)
Full Name
NFATC1 antibody: HRP
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB)
(Western blot analysis of S100A4 expression in A375 cell lysate (MBS179593).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A4 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for S100A4 )
S100A4, Monoclonal Antibody (Cat# AAA179593)
Full Name
Anti-S100A4 Rabbit Monoclonal Antibody
Gene Names
S100A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of Dishevelled 3 using anti- Dishevelled 3 antibody (MBS1750845).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Dishevelled 3 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Dishevelled 3 at approximately 78KD. The expected band size for Dishevelled 3 is at 78KD. )
Dishevelled 3, Polyclonal Antibody (Cat# AAA1750845)
Full Name
Anti-Dishevelled 3 Picoband Antibody
Gene Names
DVL3; DRS3
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat brain lysate showing detection of ~101.2 kDa NFATC1 protein using Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~101.2 kDa. Other Band(s): ~60 kDa isoform.)
NFATC1, Polyclonal Antibody (Cat# AAA8000521)
Full Name
NFATC1 antibody: ATTO 594
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of PROX1 expression in HepG2 cell lysate (MBS179781).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PROX1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PROX1 )
PROX1, Monoclonal Antibody (Cat# AAA179781)
Full Name
Anti-PROX1 Rabbit Monoclonal Antibody
Reactivity
Human
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Rat brain lysate showing detection of ~101.2 kDa NFATC1 protein using Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~101.2 kDa. Other Band(s): ~60 kDa isoform.)
NFATC1, Polyclonal Antibody (Cat# AAA8000528)
Full Name
NFATC1 antibody: Biotin
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Phospho-mTOR (S2448) expression in HEK293 cell lysate (MBS1750174).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MTOR )
mTOR, Monoclonal Antibody (Cat# AAA1750174)
Full Name
Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human, Mouse
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Rat brain lysate showing detection of ~101.2 kDa NFATC1 protein using Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~101.2 kDa. Other Band(s): ~60 kDa isoform.)
NFATC1, Polyclonal Antibody (Cat# AAA8000533)
Full Name
NFATC1 antibody: RPE
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Phospho-mTOR (S2481) expression in (1)293 cell lysate treated with LP; (2)293 cell lysate (MBS1750175).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MTOR )
mTOR, Monoclonal Antibody (Cat# AAA1750175)
Full Name
Anti-Phospho-mTOR (S2481) Rabbit Monoclonal Antibody
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Rat brain lysate showing detection of ~101.2 kDa NFATC1 protein using Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~101.2 kDa. Other Band(s): ~60 kDa isoform.)
NFATC1, Polyclonal Antibody (Cat# AAA8000518)
Full Name
NFATC1 antibody: ATTO 390
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~60.4 kDa SMAD4 protein using Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~60.4 kDa.)
SMAD4, Polyclonal Antibody (Cat# AAA8000583)
Full Name
SMAD4 Antibody: FITC
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat brain lysate showing detection of ~101.2 kDa NFATC1 protein using Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~101.2 kDa. Other Band(s): ~60 kDa isoform.)
NFATC1, Polyclonal Antibody (Cat# AAA8000529)
Full Name
NFATC1 antibody: FITC
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse kidney, using GATA2/3 Antibody(MBS179649)GATA2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GATA2 Antibody (MBS179649)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
GATA2/3, Monoclonal Antibody (Cat# AAA179649)
Full Name
Anti-GATA2/3 Rabbit Monoclonal Antibody
Gene Names
GATA2; DCML; IMD21; NFE1B; MONOMAC
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-SFRP2 Polyclonal Antibody . Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
SFRP2, Polyclonal Antibody (Cat# AAA8001580)
Full Name
SFRP2 Antibody: ATTO 390
Gene Names
SFRP2; FRP-2; SARP1; SDF-5
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of mTOR expression in Jurkat cell lysate (MBS179041).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MTOR )
mTOR/Tor, Monoclonal Antibody (Cat# AAA179041)
Full Name
Anti-mTOR/Tor Rabbit Monoclonal Antibody
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of BMAL1/ARNTL using anti- BMAL1/ARNTL antibody (MBS1750361).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: HELA whole Cell lysates,Lane 2: HEPG2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- BMAL1/ARNTL antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BMAL1/ARNTL at approximately 69KD. The expected band size for BMAL1/ARNTL is at 69KD. )
BMAL1/ARNTL, Polyclonal Antibody (Cat# AAA1750361)
Full Name
Anti-BMAL1/ARNTL Picoband Antibody
Gene Names
ARNTL; TIC; JAP3; MOP3; BMAL1; PASD3; BMAL1c; bHLHe5
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-SFRP2 Polyclonal Antibody . Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
SFRP2, Polyclonal Antibody (Cat# AAA8001588)
Full Name
SFRP2 Antibody: Alkaline Phosphatase
Gene Names
SFRP2; FRP-2; SARP1; SDF-5
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat brain lysate showing detection of ~101.2 kDa NFATC1 protein using Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~101.2 kDa. Other Band(s): ~60 kDa isoform.)
NFATC1, Polyclonal Antibody (Cat# AAA8000519)
Full Name
NFATC1 antibody: ATTO 488
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat brain lysate showing detection of ~101.2 kDa NFATC1 protein using Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~101.2 kDa. Other Band(s): ~60 kDa isoform.)
NFATC1, Polyclonal Antibody (Cat# AAA8000532)
Full Name
NFATC1 antibody: PerCP
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-SFRP2 Polyclonal Antibody . Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
SFRP2, Polyclonal Antibody (Cat# AAA8001581)
Full Name
SFRP2 Antibody: ATTO 488
Gene Names
SFRP2; FRP-2; SARP1; SDF-5
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-SFRP2 Polyclonal Antibody . Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
SFRP2, Polyclonal Antibody (Cat# AAA8001595)
Full Name
SFRP2 Antibody: RPE
Gene Names
SFRP2; FRP-2; SARP1; SDF-5
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Phospho-HSF1 (S326) expression in HeLa cell lysates treated with heat (MBS1750227).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HSF1 )
HSF1, Monoclonal Antibody (Cat# AAA1750227)
Full Name
Anti-Phospho-HSF1 (S326) Rabbit Monoclonal Antibody
Gene Names
HSF1; HSTF1
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Rat brain lysate showing detection of ~101.2 kDa NFATC1 protein using Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-NFATC1 Polyclonal Antibody (SPC-727) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~101.2 kDa. Other Band(s): ~60 kDa isoform.)
NFATC1, Polyclonal Antibody (Cat# AAA8000526)
Full Name
NFATC1 antibody: Alkaline Phosphatase
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Western Blot (WB)
(Western blot analysis of PROX1 expression in HepG2 cell lysate (MBS179782).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PROX1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PROX1 )
PROX1, Monoclonal Antibody (Cat# AAA179782)
Full Name
Anti-PROX1 Rabbit Monoclonal Antibody
Reactivity
Human
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of MEK5 expression in Hela cell lysate (MBS179996).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K5 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP2K5 )
MEK5, Monoclonal Antibody (Cat# AAA179996)
Full Name
Anti-MEK5 Rabbit Monoclonal Antibody
Gene Names
MAP2K5; MEK5; MAPKK5; PRKMK5; HsT17454
Reactivity
Human
Applications
IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of NOTCH4 expression in Mouse lung lysate (MBS179863).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOTCH4 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NOTCH4 )
NOTCH4, Monoclonal Antibody (Cat# AAA179863)
Full Name
Anti-NOTCH4 Rabbit Monoclonal Antibody
Gene Names
NOTCH4; INT3
Reactivity
Human, Mouse
Applications
FC/FACS, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of NFAT2 expression in Ramos cell lysate (MBS179276).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFATC1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NFATC1 )
NFAT2, Monoclonal Antibody (Cat# AAA179276)
Full Name
Anti-NFAT2 Rabbit Monoclonal Antibody
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2
Reactivity
Human
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~60.4 kDa SMAD4 protein using Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~60.4 kDa.)
SMAD4, Polyclonal Antibody (Cat# AAA8000587)
Full Name
SMAD4 Antibody: RPE
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~60.4 kDa SMAD4 protein using Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~60.4 kDa.)
SMAD4, Polyclonal Antibody (Cat# AAA8000571)
Full Name
SMAD4 Antibody
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-SFRP2 Polyclonal Antibody . Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
SFRP2, Polyclonal Antibody (Cat# AAA8001592)
Full Name
SFRP2 Antibody: HRP
Gene Names
SFRP2; FRP-2; SARP1; SDF-5
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~60.4 kDa SMAD4 protein using Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~60.4 kDa.)
SMAD4, Polyclonal Antibody (Cat# AAA8000586)
Full Name
SMAD4 Antibody: PerCP
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-SFRP2 Polyclonal Antibody . Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
SFRP2, Polyclonal Antibody (Cat# AAA8001594)
Full Name
SFRP2 Antibody: PerCP
Gene Names
SFRP2; FRP-2; SARP1; SDF-5
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~60.4 kDa SMAD4 protein using Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~60.4 kDa.)
SMAD4, Polyclonal Antibody (Cat# AAA8000580)
Full Name
SMAD4 Antibody: Alkaline Phosphatase
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~60.4 kDa SMAD4 protein using Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~60.4 kDa.)
SMAD4, Polyclonal Antibody (Cat# AAA8000584)
Full Name
SMAD4 Antibody: HRP
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~60.4 kDa SMAD4 protein using Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~60.4 kDa.)
SMAD4, Polyclonal Antibody (Cat# AAA8000575)
Full Name
SMAD4 Antibody: ATTO 594
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~60.4 kDa SMAD4 protein using Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~60.4 kDa.)
SMAD4, Polyclonal Antibody (Cat# AAA8000572)
Full Name
SMAD4 Antibody: ATTO 390
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-SFRP2 Polyclonal Antibody . Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
SFRP2, Polyclonal Antibody (Cat# AAA8001591)
Full Name
SFRP2 Antibody: FITC
Gene Names
SFRP2; FRP-2; SARP1; SDF-5
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~60.4 kDa SMAD4 protein using Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD4 Polyclonal Antibody (SPC-730) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~60.4 kDa.)
SMAD4, Polyclonal Antibody (Cat# AAA8000582)
Full Name
SMAD4 Antibody: Biotin
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-SFRP2 Polyclonal Antibody . Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
SFRP2, Polyclonal Antibody (Cat# AAA8001583)
Full Name
SFRP2 Antibody: ATTO 594
Gene Names
SFRP2; FRP-2; SARP1; SDF-5
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-SFRP2 Polyclonal Antibody . Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
SFRP2, Polyclonal Antibody (Cat# AAA8001590)
Full Name
SFRP2 Antibody: Biotin
Gene Names
SFRP2; FRP-2; SARP1; SDF-5
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of ETS1 using anti- ETS1 antibody (MBS1750518).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: NIH3T3 whole Cell lysates,Lane 2: A375 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- ETS1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ETS1 at approximately 54KD. The expected band size for ETS1 is at 54KD. )
ETS1, Polyclonal Antibody (Cat# AAA1750518)
Full Name
Anti-ETS1 Picoband Antibody
Gene Names
ETS1; p54; ETS-1; EWSR2; c-ets-1
Reactivity
Human, Mouse
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Rabbit Anti-SFRP2 Polyclonal Antibody . Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-SFRP2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.)
SFRP2, Polyclonal Antibody (Cat# AAA8001579)
Full Name
SFRP2 Antibody
Gene Names
SFRP2; FRP-2; SARP1; SDF-5
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
MYOCD / Myocardin, Recombinant Protein (Cat# AAA2901849)
Full Name
Human MYOCD / Myocardin Recombinant Protein
Gene Names
MYOCD; MYCD
Applications
EIA, WB, AP
Purity
>95% by SDS-PAGE
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Phospho-DDR1-Y792 antibody at 1:1000 dilution. 293T cells were treated by ATP(5 mM) at 30℃ for 1 hour. NIH/3T3 cells were treated by ATP(5 mM) at 30℃ for 1 hour. C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Phospho-DDR1-Y792, Polyclonal Antibody (Cat# AAA9143847)
Full Name
Phospho-DDR1-Y792 Rabbit pAb
Gene Names
DDR1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
ETS2, Polyclonal Antibody (Cat# AAA9142485)
Full Name
ETS2 Rabbit pAb
Gene Names
ETS2; ETS2IT1
Reactivity
Mouse, Rat
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
