Mouse NF200 Monoclonal Antibody | anti-NEFH antibody

Anti-NF200 antibody (monoclonal)

Cat. Num. AAA175078

• Gene Names: NEFH; NFH
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry
• Synonyms: NF200; Monoclonal Antibody; Anti-NF200 antibody (monoclonal); Neurofilament heavy polypeptide; neurofilament; heavy polypeptide; NFH; KIAA0845; 200 kDa neurofilament protein; Neurofilament triplet H protein; anti-NEFH antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: N52
• Specificity: No cross reactivity with other proteins.
• Form/Format: Lyophilized
• Sequence Length: 1026

• Applicable Applications for anti-NEFH antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin
• Application Notes: Western Blot ; Concentration : 0.5 ug/mL; Tested Species : Human,Mouse, Rat ; ; Immunohistochemistry (Paraffin-embedded Section); Concentration: 1-2 ug/mL; Tested Species: Human, Mouse, Rat ; Antigen Retrieval: By Heat; ; Immunohistochemistry (Frozen Section) ; Concentrarion: 1-2 ug/mL; Tested Species: Human, Mouse, Rat ; ; ; Other applications have not been tested.; ; Optimal dilutions should be determined by end users.
• Immunogen: C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.
• Contents: Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
• Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
• Preparation and Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for 6 months. Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-NF200 antibody (monoclonal), MBS175078, IHC(P)IHC(P): Rat Brain Tissue )

Related Product Information for anti-NEFH antibody

Background: Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD(NFL), 125 kD(NFM), and 200 kD(NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein(NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions(exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease(CMT).

NCBI and Uniprot Product Information

• NCBI GI #: 226726294
• NCBI GeneID: 4744
• UniProt Accession #: P12036; Q96HF8; Q9UJS7; Q9UQ14; B4DYY4
• NCBI Official Full Name: Neurofilament heavy polypeptide
• NCBI Official Synonym Full Names: neurofilament, heavy polypeptide
• NCBI Official Symbol: NEFH
• NCBI Official Synonym Symbols: NFH
• NCBI Protein Information: neurofilament heavy polypeptide; NF-H; 200 kDa neurofilament protein; neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa
• UniProt Protein Name: Neurofilament heavy polypeptide
• Protein Family: Neurofilament heavy polypeptide
• UniProt Gene Name: NEFH
• UniProt Synonym Gene Names: KIAA0845; NFH; NF-H
• UniProt Entry Name: NFH_HUMAN

NCBI Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

NFH: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. Belongs to the intermediate filament family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: mitochondrion; axon; cytoplasm; neurofilament

Molecular Function: protein binding, bridging; dynein binding; structural constituent of cytoskeleton; kinesin binding; microtubule binding; structural molecule activity; protein kinase binding

Biological Process: axonogenesis; neurofilament bundle assembly; microtubule cytoskeleton organization and biogenesis; cell projection biogenesis; peripheral nervous system neuron axonogenesis

Disease: Amyotrophic Lateral Sclerosis 1