Mouse NEFH / NF-H Monoclonal Antibody | anti-NEFH antibody

Mouse Monoclonal [clone RNF404] (IgG2a) to Human NEFH / NF-H

Cat. Num. AAA245828

• Gene Names: NEFH; NFH
• Reactivity: Bovine, Chicken, Dog, Xenopus, Guinea pig, Human, Monkey, Mouse, Rabbit, Rat, Sheep
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified
• Synonyms: NEFH / NF-H; Monoclonal Antibody; Mouse Monoclonal [clone RNF404] (IgG2a) to Human NEFH / NF-H; Anti-NEFH / NF-H Antibody (clone RNF404) IHC-plus; NEFH; 200 kDa neurofilament protein; KIAA0845; NFH; NF-H; Human NEFH; anti-NEFH antibody

• Host: Mouse
• Reactivity: Bovine, Chicken, Dog, Xenopus, Guinea pig, Human, Monkey, Mouse, Rabbit, Rat, Sheep
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: RNF404
• Specificity: Reacts exclusively with the phosphorylated isoform of the 200 kD neurofilament protein.
• Purity/Purification: Purified
• Form/Format: PBS containing 0.09% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1020

• Applicable Applications for anti-NEFH antibody: Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB)
• Application Notes: IHC-Fr (1:50 - 1:100), IHC-P (10 ug/ml), WB (1:100 - 1:500)
• Target Species: Human
• Immunogen Description: Neurofilament preparation of calf brain tissue
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-NEFH antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 32483416
• NCBI GeneID: 4744
• NCBI Accession #: NP_066554.2
• NCBI GenBank Nucleotide #: NM_021076.3
• UniProt Accession #: P12036; Q96HF8; Q9UJS7; Q9UQ14; B4DYY4
• Molecular Weight: 105,639 Da
• NCBI Official Full Name: neurofilament heavy polypeptide
• NCBI Official Synonym Full Names: neurofilament, heavy polypeptide
• NCBI Official Symbol: NEFH
• NCBI Official Synonym Symbols: NFH
• NCBI Protein Information: neurofilament heavy polypeptide; NF-H; 200 kDa neurofilament protein; neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa
• UniProt Protein Name: Neurofilament heavy polypeptide
• Protein Family: Neurofilament heavy polypeptide
• UniProt Gene Name: NEFH
• UniProt Synonym Gene Names: KIAA0845; NFH; NF-H
• UniProt Entry Name: NFH_HUMAN

NCBI Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

NFH: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. Belongs to the intermediate filament family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: mitochondrion; axon; cytoplasm; neurofilament

Molecular Function: protein binding, bridging; dynein binding; structural constituent of cytoskeleton; kinesin binding; microtubule binding; structural molecule activity; protein kinase binding

Biological Process: axonogenesis; neurofilament bundle assembly; microtubule cytoskeleton organization and biogenesis; cell projection biogenesis; peripheral nervous system neuron axonogenesis

Disease: Amyotrophic Lateral Sclerosis 1