Mouse Neuroligin-1 Monoclonal Antibody | anti-Nlgn1 antibody

Neuroligin-1 Monoclonal Antibody

Cat. Num. AAA191335

• Applications: Immunoblot, Western Blot, Immunofluorescence
• Synonyms: Neuroligin-1; Monoclonal Antibody; Neuroligin-1 Monoclonal Antibody; anti-Nlgn1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: This antibody recognizes human, mouse, and rat Neuroligin-1. It does not crossreact with other Neuroligins.
• Form/Format: 100ug (1mg/ml) Protein G-purified antibody in PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.
• Sequence Length: 843

• Applicable Applications for anti-Nlgn1 antibody: Immunoblot, Immunofluorescence (IF)
• Application Notes: Immunoblot: 1-5ug/ml. A band of ~120kDa is detected.; IF: 10ug/ml; Dilute in PBS or medium that is identical to that used in the assay system
• Immunogen: Fusion protein corresponding to aa 718-843 (cytoplasmic C-terminus) of rat Neuroligin1, accession no. Q62765). This sequence is 99% identical to mouse and 99% identical to human Neuroligin-1.
• Preparation and Storage: This product is stable for at least one (1) year at-20 degree C.

Testing Data

(Detection of Neuroligin-1 in mouse brain lysate at 5ug/ml. )

Testing Data

(Detection of Neuroligin-1 in neuroblastoma cell line SK-N-BE at 10ug/ml: DAPI (blue) nuclear stain, Texas Red F actin stain, FITC (green) Neuroligin-1 stain.)

Related Product Information for anti-Nlgn1 antibody

Neuroligin-1 is a neuronal cell surface protein and member of the type-B carboxylesterase/lipase family. It is a necessary component in the maturation of excitatory synapses, it participates in regulation of synaptic plasticity, and it is important in the development of long-term memory in adult mammalian amygdala. It's participation in cell-cell interactions may occur through assembly of intracellular junctions by binding of beta-neurexins. In nervous system tissue, Neuroligin-1 is observed primarily in neurons and in the spinal cord.

NCBI and Uniprot Product Information

• NCBI GI #: 16758736
• NCBI GeneID: 116647
• NCBI Accession #: NP_446320.1
• NCBI GenBank Nucleotide #: NM_053868.2
• UniProt Accession #: Q62765
• Molecular Weight: 90,995 Da
• NCBI Official Full Name: neuroligin-1
• NCBI Official Synonym Full Names: neuroligin 1
• NCBI Official Symbol: Nlgn1
• NCBI Protein Information: neuroligin-1
• UniProt Protein Name: Neuroligin-1
• Protein Family: Neuroligin
• UniProt Gene Name: Nlgn1

NCBI Description

transmembrane ligand for neurexins; involved in development and maturation of synaptic connections [RGD, Feb 2006]

Uniprot Description

Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Required to maintain wakefulness quality and normal synchrony of cerebral cortex activity during wakefulness and sleep ().