Rabbit anti-Human MAP Kinase 15 Polyclonal Antibody | anti-MAPK15 antibody

MAP Kinase 15, NT (Mitogen-activated Protein Kinase 15, MAPK 15, MAPK15, Extracellular Signal-regulated Kinase 7, ERK7, ERK-7, Extracellular Signal-regulated Kinase 8, ERK8, ERK-8) (APC)

Cat. Num. AAA6486793

• Gene Names: MAPK15; ERK7; ERK8
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MAP Kinase 15; Polyclonal Antibody; NT (Mitogen-activated Protein Kinase 15; MAPK 15; MAPK15; Extracellular Signal-regulated Kinase 7; ERK7; ERK-7; Extracellular Signal-regulated Kinase 8; ERK8; ERK-8) (APC); EC=2.7.11.24; anti-MAPK15 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ERK8.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-MAPK15 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human ERK8 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ERK8 (arrow) using rabbit polyclonal ERK8 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK15 gene (Lane 2) (Origene Technologies).)

Western Blot (WB)

(Western blot analysis of anti-ERK8 Antibody (N-term) in T47D cell line lysates (35ug/lane). ERK8 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ERK8 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-MAPK15 antibody

Antibodies; Protein Kinases

References

1. Saelzler, M.P., J. Biol. Chem. 281(24), 16821-16832 (2006). 2. Iavarone, C., J. Biol. Chem. 281(15), 10567-10576 (2006). 3. Klevernic, I.V., Biochem. J. 394(PT 1), 365-373 (2006). 4. Suzuki, Y., Genome Res. 14(9), 1711-1718 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 95147356
• NCBI GeneID: 225689
• NCBI Accession #: NP_620590
• NCBI GenBank Nucleotide #: NM_139021
• UniProt Accession #: Q8TD08; Q2TCF9; Q8N362
• Molecular Weight: 31,112 Da
• NCBI Official Full Name: mitogen-activated protein kinase 15
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 15
• NCBI Official Symbol: MAPK15
• NCBI Official Synonym Symbols: ERK7; ERK8
• NCBI Protein Information: mitogen-activated protein kinase 15; ERK-7; ERK-8; MAP kinase 15; MAPK 15; extracellular regulated kinase 8 delta; extracellular signal regulated kinase 8; extracellular signal-regulated kinase 7; extracellular signal-regulated kinase 8
• UniProt Protein Name: Mitogen-activated protein kinase 15
• UniProt Gene Name: MAPK15
• UniProt Synonym Gene Names: ERK7; ERK8; MAP kinase 15; MAPK 15; ERK-7; ERK-8
• UniProt Entry Name: MK15_HUMAN

Uniprot Description

ERK7: In vitro, phosphorylates MBP. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; CMGC group; MAPK family; Erk7 subfamily

Chromosomal Location of Human Ortholog: -

Cellular Component: extracellular region; intracellular; nucleus

Molecular Function: MAP kinase activity; SH3 domain binding; ATP binding

Biological Process: negative regulation of DNA replication; positive regulation of protein ubiquitination; protein amino acid autophosphorylation; positive regulation of protein catabolic process; MAPKKK cascade; negative regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; response to estradiol stimulus