Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded Cerebellum stained with Neurofilament Monoclonal Antibody (SPM204).)
Mouse Neurofilament (NF-L) Monoclonal Antibody | anti-NEFL antibody
Neurofilament (NF-L) (Neuronal Marker) Mouse Monoclonal Antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded Cerebellum stained with Neurofilament Monoclonal Antibody (SPM204).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded Cerebellum stained with Neurofilament Monoclonal Antibody (SPM204).)
NCBI and Uniprot Product Information
NCBI Description
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
Uniprot Description
NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F).
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 8p21
Cellular Component: axon; cytoplasm; cytosol; neurofilament; TSC1-TSC2 complex
Molecular Function: identical protein binding; protein binding; protein binding, bridging; protein C-terminus binding; Ras guanyl-nucleotide exchange factor activity; structural constituent of cytoskeleton
Biological Process: anterograde axon cargo transport; axon transport of mitochondrion; intermediate filament organization; MAPKKK cascade; neurofilament bundle assembly; retrograde axon cargo transport
Disease: Charcot-marie-tooth Disease, Axonal, Type 2e; Charcot-marie-tooth Disease, Demyelinating, Type 1f
Research Articles on NEFL
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Product Notes
The NEFL nefl (Catalog #AAA439586) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Neurofilament (NF-L) (Neuronal Marker) Mouse Monoclonal Antibody reacts with Human, Rat, Pig, Cow, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Neurofilament (NF-L) can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Formalin. Immunofluorescence (1-2ug/ml) Flow Cytometry (0.5-1ug/million cells in 0.1ml) Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the NEFL nefl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neurofilament (NF-L), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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