Human Neurofilament light polypeptide ELISA Kit | NEFL elisa kit

Human Neurofilament light polypeptide ELISA Kit

Cat. Num. AAA765857

• Gene Names: NEFL; NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110
• Reactivity: Human
• Synonyms: Neurofilament light polypeptide; Human Neurofilament light polypeptide ELISA Kit; NEFL/Neurofilament light polypeptide/NF-L/68 kDa neurofilament protein/Neurofilament triplet L protein; NEFL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NEFL. No significant cross-reactivity or interference between NEFL and analogues was observed.
• Sequence Length: 543

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NEFL elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 105990539
• NCBI GeneID: 4747
• NCBI Accession #: NP_006149.2
• NCBI GenBank Nucleotide #: NM_006158.4
• Molecular Weight: 61,517 Da
• NCBI Official Full Name: neurofilament light polypeptide
• NCBI Official Synonym Full Names: neurofilament, light polypeptide
• NCBI Official Symbol: NEFL
• NCBI Official Synonym Symbols: NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110
• NCBI Protein Information: neurofilament light polypeptide
• UniProt Protein Name: Neurofilament light polypeptide
• Protein Family: Neurofilament light polypeptide
• UniProt Gene Name: NEFL
• UniProt Synonym Gene Names: NF68; NFL; NF-L
• UniProt Entry Name: NFL_HUMAN

NCBI Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

Uniprot Description

NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F).

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: axon; cytoplasm; cytosol; growth cone; myelin sheath; neurofilament; TSC1-TSC2 complex

Molecular Function: identical protein binding; phospholipase binding; protein binding; protein binding, bridging; protein C-terminus binding; protein domain specific binding; protein heterodimerization activity; structural constituent of cytoskeleton

Biological Process: activation of MAPKK activity; anterograde axon cargo transport; axon guidance; axon regeneration in the peripheral nervous system; axon transport of mitochondrion; cerebral cortex development; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; hippocampus development; innate immune response; insulin receptor signaling pathway; intermediate filament organization; intermediate filament polymerization and/or depolymerization; MAPKKK cascade; microtubule cytoskeleton organization and biogenesis; negative regulation of neuron apoptosis; nerve growth factor receptor signaling pathway; neurofilament bundle assembly; neuromuscular process controlling balance; positive regulation of axonogenesis; protein polymerization; Ras protein signal transduction; regulation of axon diameter; response to corticosterone stimulus; response to toxin; retrograde axon cargo transport; small GTPase mediated signal transduction; spinal cord development; synaptic transmission; vascular endothelial growth factor receptor signaling pathway

Disease: Charcot-marie-tooth Disease, Axonal, Type 2e; Charcot-marie-tooth Disease, Demyelinating, Type 1f