Testing Data
(Schematic antibody structure of the recombinant antibody Netrin-1.. The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a human IgG2 Fc region.)
Human anti-Human, Mouse Netrin-1 Monoclonal Antibody | anti-NTN1 antibody
Human Anti-Netrin-1 (NTN1)
Reactivity
Human, Mouse
Applications
ELISA
Purity
Purified by Protein A affinity chromatography.
Synonyms
Netrin-1; Monoclonal Antibody; Human Anti-Netrin-1 (NTN1); anti-NTN1 antibody
Host
Human
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2, l
Clone Number
2F5
Specificity
Recognizes human Netrin-1. Does not recognize Netrin-4. Blocks human and mouse Netrin-1 activity on tumor cell lines in vitro and in vivo. Inhibits Netrin-1-enhanced infectivity of Hepatitis C Virus particles. Species Crossreactivity: mouse
Purity/Purification
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 10% glycerol. No preservative added.
Applicable Applications for anti-NTN1 antibody
ELISA (EIA)
Application Notes
Blocking: Inhibits the activation of human and mouse Netrin-1 to human or mouse receptors DCC or UNC5 (KD antibody-Netrin-1 is 1.5nM). Optimal dilutions to be determined by the researcher.
Immunogen
Recombinant protein corresponding to human Netrin-1 expressed in HEK 293 cells.
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(Schematic antibody structure of the recombinant antibody Netrin-1.. The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a human IgG2 Fc region.)
Testing Data
(Schematic antibody structure of the recombinant antibody Netrin-1.. The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a human IgG2 Fc region.)
Testing Data
(Tumor cells (OCI-Ly3) were implanted in SCID mice by subcutaneous injection of 3x10e6 cells in 100ul of PBS. When tumors reached 150mm3, mice received intraperitoneal injections of blocking Netrin-1 (2F5) at 20mg/kg or an equal volume of the antibody control Netrin-1 (H4) (preservative free) every two days. Tumor growth rates from the beginning of treatment are shown.)
Testing Data
(Tumor cells (OCI-Ly3) were implanted in SCID mice by subcutaneous injection of 3x10e6 cells in 100ul of PBS. When tumors reached 150mm3, mice received intraperitoneal injections of blocking Netrin-1 (2F5) at 20mg/kg or an equal volume of the antibody control Netrin-1 (H4) (preservative free) every two days. Tumor growth rates from the beginning of treatment are shown.)
Related Product Information for anti-NTN1 antibody
Netrin-1 controls guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Netrin-1 is also involved in tumorigenesis by regulating apoptosis. Netrin-1 promotes atherosclerosis by retaining macrophages in the artery wall. Netrin-1 also governs induced pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by limiting apoptosis mediated by Netrin-1 receptors DCC or UNC5b.
References
1. Epidermal Growth Factor Receptor-Dependent Mutual Amplification between Netrin-1 and the Hepatitis C Virus: M.L. Plissonnier, et al.; PLoS Biol. 14, e1002421 (2016). 2. Targeting netrin-1/DCC interaction in diffuse large B-cell and mantle cell lymphomas: T. Broutier, et al.; EMBO Mol. Med. 8, 96 (2016). 3. Netrin-1 regulates somatic cell reprogramming and pluripotency maintenance: D. Ozmandenci, et al.; Nat. Commun. 6, 7398 (2015).
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Product Notes
The NTN1 (Catalog #AAA6120624) is an Antibody produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Human Anti-Netrin-1 (NTN1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Netrin-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Blocking: Inhibits the activation of human and mouse Netrin-1 to human or mouse receptors DCC or UNC5 (KD antibody-Netrin-1 is 1.5nM). Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the NTN1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Netrin-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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