Netrin 1 Positive Control | NTN1 positive control

Netrin 1 Antibody Western Blot Positive Control

Cat. Num. AAA543004

• Gene Names: NTN1; NTN1L
• Applications: Western Blot
• Synonyms: Netrin 1; Netrin 1 Antibody Western Blot Positive Control; epididymis tissue protein Li 131P; mouse; homolog of; Netrin 1-like; Ntn 1; Unc6 antibody; NTN1 positive control

• Form/Format: Western Blot Positive Control
• Concentration: Empirically set for optimal signal-to-noise ratio (varies by lot)
• Sequence Length: 604

• Applicable Applications for NTN1 positive control: Western Blot (WB)
• Application Notes: Western Blot: 1:500
• Component: Purified Protein
• Expression: highest levels in heart, small intestine, colon, liver and prostate. Reduced expression in brain tumors and neuroblastomas. Expressed in epididymis (at protein level)
• Buffer: Prepared in SDS-sample buffer
• Subcellular Location: Extracellular matrix, Secreted
• Preparation and Storage: Store at -20°C for long term storage

Western Blot

(Western Blot of Netrin1 (MBS543004) antibody with PC-NTRN samples. Antibody Dilution 1:500 in DiluObufferDilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample.)

Related Product Information for NTN1 positive control

Western Blot Positive Control Netrin-1 precursor Antibody
Netrins control guidance of CNS commissural axons and peripheral motor axons;serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis;Involved in tumorigenesis by regulating apoptosis.

Netrin-1 (NTRN1) also known as axonal guidance protein (NTRN1) is a diffuse protein made by the floor plate cells that belongs to the Netrirt family of proteins that guides axons to or away from the midline of the developing nervous system (1). ln mammals, netrins are ligands for DCC receptor and the UNCS receptor homologues. Netrin-'1 , located at human chromosome 17p13-p12, attracts spinal commissural axons and repels trochlear axons in vitro. ln mice, deficlencies in Netrin-1 led to defects in spinal commissural axon projections (2). ln the dorsal and intermediate spinal cord, it prevents DRG axons from being directed towards the dorsal spinal cord (3). Netrin-1 and its associated receptors play a key role cell death and tumor formation. Netrin-1/receptor pair induces apoptosis in cultured cells and also acts as regulators of tumor development (4). These properties of Netrin-1 have been implicated in colorectal tumorigenesis by regulating apoptosis (5). ln the embryo, Netrin-1 is widely exDressed in the developing nervous system and in mesodermal tissues. ln adults, it is highly expressed in heart, small intestine, colon, liver and prostrate. It is also expressed in brain tumors and neuroblastomas. Netrin-1 is approximately a 73kDa protein. The Netrinl-selective antibody (MBS540531) was generated against a peptide near C-terminal end of the Netrinl protein using cyclic peptide methodology for generating antibodies, which results in higher titer and specificity (6). MBS540531 was used to develop FITC-conjugated NTRNl-selective antibodies (MBS540531). Western blot positive control samples and antigenic blocklng peptide are available. The affinity purified mono-specific polyclonal antibody to NTRNl strongly labels a 73kDa proteln in western blot positive control (MBS543004) samples and invarious tissues examined. Antibodies can be conjugated to secondary enzymes or fluorescent probes upon request as an additional charge.

NCBI and Uniprot Product Information

• NCBI GI #: 148613884
• NCBI GeneID: 9423
• NCBI Accession #: NP_004813.2
• NCBI GenBank Nucleotide #: NM_004822.2
• UniProt Accession #: O95631; E9KL51
• NCBI Official Full Name: netrin-1
• NCBI Official Synonym Full Names: netrin 1
• NCBI Official Symbol: NTN1
• NCBI Official Synonym Symbols: NTN1L
• NCBI Protein Information: netrin-1
• UniProt Protein Name: Netrin-1
• Protein Family: Netrin
• UniProt Gene Name: NTN1
• UniProt Synonym Gene Names: NTN1L
• UniProt Entry Name: NET1_HUMAN

NCBI Description

Netrin is included in a family of laminin-related secreted proteins. The function of this gene has not yet been defined; however, netrin is thought to be involved in axon guidance and cell migration during development. Mutations and loss of expression of netrin suggest that variation in netrin may be involved in cancer development. [provided by RefSeq, Jul 2008]

Uniprot Description

NTN1: Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: basement membrane; cytoplasm; extracellular region

Molecular Function: protein binding

Biological Process: anterior/posterior axon guidance; apoptosis; axon guidance; Cdc42 protein signal transduction; cell-cell adhesion; establishment of nucleus localization; inner ear morphogenesis; negative regulation of axon extension; neuron migration; positive regulation of axon extension; positive regulation of cell proliferation; Ras protein signal transduction; regulation of cell migration; substrate-bound cell migration, cell extension