Immunofluorescence (IF)
(Immunofluorescent detection of p75NTR in cultured rat dorsal root ganglion (DRG) using MBS607731. Merged pictures of cultured rat DRG triple-stained for p75 NTR (red color), beta-Tubulin (green color) and nuclei using DAPI (violet color).)
Mouse anti-Rat Nerve Growth Factor Receptor, p75NTR Monoclonal Antibody | anti-ngfr antibody
Nerve Growth Factor Receptor, p75NTR (Low-affinity, NGF receptor, Neurotrophin receptor, Gp80-LNGFR, p75 ICD)
Gene Names
ngfr; nrh1; CD271; NRH1b; p75ntr; p75(NTR); tnfrsf16; MGC131034; Gp80-LNGFR
Reactivity
Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Nerve Growth Factor Receptor; p75NTR; Monoclonal Antibody; p75NTR (Low-affinity; NGF receptor; Neurotrophin receptor; Gp80-LNGFR; p75 ICD); Anti -Nerve Growth Factor Receptor; anti-ngfr antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
IgG
Clone Number
9G396
Specificity
Recognizes the extracellular domain of the neurotrophin receptor in p75NTR-expressing cells in rat spinal cord and brain. Does not cross react with p75NTR-expressing cells in other species.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder, 1% BSA. Reconstitute with 100ul sterile ddH2O.
Applicable Applications for anti-ngfr antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Western Blot, and Immunohistochemistry and immunofluorescence.
Dilution: Immunohistochemistry: 1-2ug/ml stains paraformaldehyde-fixed, acetone-fixed and paraffin-embedded sections of rat spinal cord and brain.
Dilution: Immunohistochemistry: 1-2ug/ml stains paraformaldehyde-fixed, acetone-fixed and paraffin-embedded sections of rat spinal cord and brain.
Immunogen
Synthetic peptide corresponding to rat NGF receptor. Cellular Location: Membrane; single-pass type I membrane protein.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunofluorescence (IF)
(Immunofluorescent detection of p75NTR in cultured rat dorsal root ganglion (DRG) using MBS607731. Merged pictures of cultured rat DRG triple-stained for p75 NTR (red color), beta-Tubulin (green color) and nuclei using DAPI (violet color).)
Immunofluorescence (IF)
(Immunofluorescent detection of p75NTR in cultured rat dorsal root ganglion (DRG) using MBS607731. Merged pictures of cultured rat DRG triple-stained for p75 NTR (red color), beta-Tubulin (green color) and nuclei using DAPI (violet color).)
Immunofluorescence (IF)
(Immunofluorescent detection of p75NTR in cultured rat dorsal root ganglion (DRG). (A) Detection of p75NTR using MBS607731. (B) Detection of betaIII-Tubulin. (C) DAPI staining. (D) Merged A, B and C.)
Immunofluorescence (IF)
(Immunofluorescent detection of p75NTR in cultured rat dorsal root ganglion (DRG). (A) Detection of p75NTR using MBS607731. (B) Detection of betaIII-Tubulin. (C) DAPI staining. (D) Merged A, B and C.)
Immunohistochemistry (IHC)
(IHC staining of p75NTR in rat forebrain using MBS607731. Paraformaldehyde fixed rat brain sections were incubated with 1ug/ml antibody overnight, followed by incubation with biotinylated rabbit anti-mouse IgG conjugate. The immunocomplex was then visualized with DAB . Neurons, in the septal nuclei of basal forebrain, known to be cholinergic neurons, show strong staining for p75NTR.)
Immunohistochemistry (IHC)
(IHC staining of p75NTR in rat forebrain using MBS607731. Paraformaldehyde fixed rat brain sections were incubated with 1ug/ml antibody overnight, followed by incubation with biotinylated rabbit anti-mouse IgG conjugate. The immunocomplex was then visualized with DAB . Neurons, in the septal nuclei of basal forebrain, known to be cholinergic neurons, show strong staining for p75NTR.)
Related Product Information for anti-ngfr antibody
Nerve Growth Factor Receptor, p75NTR, generated by Chandlers et al. p75NTR, was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons.
Product Categories/Family for anti-ngfr antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
nerve growth factor receptor
NCBI Official Synonym Full Names
nerve growth factor receptor<
NCBI Official Symbol
ngfr
NCBI Official Synonym Symbols
nrh1; CD271; NRH1b; p75ntr; p75(NTR); tnfrsf16; MGC131034; Gp80-LNGFR
NCBI Protein Information
nerve growth factor receptor; NRH; nerve growth factor receptor (TNFR superfamily, member 16)
Protein Family
Research Articles on ngfr
Similar Products
Product Notes
The ngfr (Catalog #AAA607731) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Nerve Growth Factor Receptor, p75NTR (Low-affinity, NGF receptor, Neurotrophin receptor, Gp80-LNGFR, p75 ICD) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Nerve Growth Factor Receptor, p75NTR can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in ELISA, Western Blot, and Immunohistochemistry and immunofluorescence. Dilution: Immunohistochemistry: 1-2ug/ml stains paraformaldehyde-fixed, acetone-fixed and paraffin-embedded sections of rat spinal cord and brain. Researchers should empirically determine the suitability of the ngfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nerve Growth Factor Receptor, p75NTR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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