Human TNFRSF16/NGFR PicoKine ELISA Kit | NGFR elisa kit

Human TNFRSF16/NGFR PicoKine ELISA Kit

Cat. Num. AAA177642

• Gene Names: NGFR; CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
• Reactivity: Human
• Synonyms: TNFRSF16/NGFR PicoKine; Human TNFRSF16/NGFR PicoKine ELISA Kit; Tumor necrosis factor receptor superfamily member 16; CD271; CD271 antigen; Gp80 LNGFR; Gp80-LNGFR; Low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor; Nerve growth factor receptor; Nerve growth factor receptor TNFR superfamily member 16; NGF receptor; NGFR; p75 ICD; p75 Neurotrophin receptor; p75 NTR; p75(NTR); p75NTR; TNFR Superfamily Member 16; TNFRSF16; TNR16_HUMAN; nerve growth factor receptor; NGFR elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human TNFRSF16
• Sequence Length: 427

• Assay Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of human TNFRSF16 in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
• Immunogen: sf21, K29-N250
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human TNFRSF16 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human TNFRSF16 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|81|330|1560|85|360|1350; Standard deviation|4.21|21.45|90.48|5.27|27|90.45; CV(%)|5.2|6.5|5.8|6.2|7.5|6.7
• Sample Data: Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000; O.D.|0.009|0.057|0.129|0.205|0.416|0.685|1.375|2.203
• Anticoagulant: Heparin, EDTA or Citrate
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human TNFRSF16/NGFR PicoKine ELISA Kit standard curve )

Related Product Information for NGFR elisa kit

Principle of the assay: Human TNFRSF16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF16 has been precoated onto 96-well plates. Standards (sf21, K29 - N250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF16 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFRSF16 amount of sample captured in plate.

Background: Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5. The nerve growth factor receptor gene is at human chromosome region 17q12-17q22, distal to the chromosome 17 breakpoint in acute leukemias. The neurotrophin receptor p75(NTR), a tumor necrosis factor receptor superfamily member expressed in hepatic stellate cells after fibrotic and cirrhotic liver injury in humans, is a regulator of liver repair. In mice, depletion of p75(NTR) exacerbated liver pathology and inhibited hepatocyte proliferation in vivo. In addition, it is showed that neurotrophins activate p75(NTR) to induce apoptosis through the induction of the sphingomyelin (SM) cycle and increased production of ceramide. Overexpression of p75(NTR) is also found to activate the SM pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 4505393
• NCBI GeneID: 4804
• NCBI Accession #: NP_002498.1
• NCBI GenBank Nucleotide #: NM_002507.3
• UniProt Accession #: P08138; B2R961; B4E096
• Molecular Weight: 35,767 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 16
• NCBI Official Synonym Full Names: nerve growth factor receptor
• NCBI Official Symbol: NGFR
• NCBI Official Synonym Symbols: CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 16
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 16
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: NGFR
• UniProt Synonym Gene Names: TNFRSF16; NGF receptor
• UniProt Entry Name: TNR16_HUMAN

NCBI Description

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq, Jul 2008]

Uniprot Description

NGFR: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Homodimer; disulfide-linked. Interacts with p75NTR- associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3. Interacts with KIDINS220 and NTRK1. Can form a ternary complex with NTRK1 and KIDINS220 and this complex is affected by the expression levels of KIDINS220. An increase in KIDINS220 expression leads to a decreased association of NGFR and NTRK1. Interacts with NTRK2; may regulate the ligand specificity of the NTRK2 receptor. Interacts with LINGO1.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 17q21-q22

Cellular Component: cell surface; clathrin-coated endocytic vesicle; cytosol; endosome; external side of plasma membrane; extracellular region; Golgi apparatus; integral to plasma membrane; lipid raft; neuron projection; nuclear envelope; nucleoplasm; nucleus; perikaryon; plasma membrane; rough endoplasmic reticulum

Molecular Function: beta-amyloid binding; nerve growth factor binding; neurotrophin TRKA receptor binding; protein binding; Rab GTPase binding; receptor activity; signal transducer activity; transmembrane receptor activity; tumor necrosis factor receptor activity; ubiquitin protein ligase binding

Biological Process: axon guidance; caspase activation; circadian regulation of gene expression; glucose homeostasis; immune response; inflammatory response; intracellular protein transport; membrane protein intracellular domain proteolysis; negative regulation of apoptosis; negative regulation of axonogenesis; negative regulation of caspase activity; negative regulation of mitochondrial depolarization; negative regulation of neuron apoptosis; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; positive regulation of axonogenesis; positive regulation of MAPKKK cascade; positive regulation of myelination; positive regulation of protein kinase B signaling cascade; positive regulation of Rho protein signal transduction; positive regulation of synaptic transmission, cholinergic; positive regulation of synaptic transmission, glutamatergic; regulation of apoptosis; regulation of caspase activity; regulation of cell proliferation; response to lipopolysaccharide; response to wounding; sensory perception of pain; tumor necrosis factor-mediated signaling pathway