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Immunofluorescence (IF) (Immunofluorescence analysis of A549 cells, using GRAH Antibody. The picture on the right is blocked with the synthesized peptide.)

Rabbit anti-Human GRAH Polyclonal Antibody | anti-GRAH antibody

GRAH Antibody

Gene Names
GZMH; CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2
Reactivity
Human
Applications
Western Blot, Immunofluorescence, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
GRAH; Polyclonal Antibody; GRAH Antibody; Granzyme H; Cytotoxic T-lymphocyte proteinase; Cathepsin G-like 2; CTSGL2; CCP-X; Cytotoxic serine protease C; CSP-C; GZMH; CGL2; anti-GRAH antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
GRAH Antibody detects endogenous levels of total GRAH protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
115
Applicable Applications for anti-GRAH antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:5000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human GRAH.
Immunogen Range
51-100
Preparation and Storage
Stable at -20 degree C for at least 1 year.

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cells, using GRAH Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunofluorescence (IF) (Immunofluorescence analysis of A549 cells, using GRAH Antibody. The picture on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of lysates from K562 cells, using GRAH Antibody. The lane on the right is blocked with the synthesized peptide.)

Western Blot (WB) (Western blot analysis of lysates from K562 cells, using GRAH Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27 kDa
NCBI Official Full Name
granzyme H isoform 3
NCBI Official Synonym Full Names
granzyme H
NCBI Official Symbol
GZMH
NCBI Official Synonym Symbols
CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2
NCBI Protein Information
granzyme H
UniProt Protein Name
Granzyme H
Protein Family
UniProt Gene Name
GZMH
UniProt Synonym Gene Names
CGL2; CTSGL2; CTSGL2; CSP-C

NCBI Description

This gene encodes a member of the peptidase S1 family of serine proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a chymotrypsin-like protease. This protein is reported to be constitutively expressed in the NK (natural killer) cells of the immune system and may play a role in the cytotoxic arm of the innate immune response by inducing target cell death and by directly cleaving substrates in pathogen-infected cells. This gene is present in a gene cluster with another member of the granzyme subfamily on chromosome 14. [provided by RefSeq, Nov 2015]

Uniprot Description

Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Participates in the antiviral response via direct cleavage of several proteins essential for viral replication.

Research Articles on GRAH

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Product Notes

The GRAH gzmh (Catalog #AAA9505811) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GRAH Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GRAH can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:5000. Researchers should empirically determine the suitability of the GRAH gzmh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRAH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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