NCAM / CD56 Monoclonal Antibody | anti-NCAM / CD56 antibody

Anti-NCAM / CD56 Antibody (aa20-718, clone 1G4) IHC-plus

Cat. Num. AAA249302

• Gene Names: NCAM1; CD56; NCAM; MSK39
• Reactivity: Mouse, Dog, Rat, Human, Monkey
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity purified
• Synonyms: NCAM / CD56; Monoclonal Antibody; Anti-NCAM / CD56 Antibody (aa20-718; clone 1G4) IHC-plus; NCAM1; CD56; CD56 antigen; MSK39; NCAM-1; N-CAM-1; NCAM; anti-NCAM / CD56 antibody

• Host: NCAM/CD56 antibody was produced in Mouse
• Reactivity: Mouse, Dog, Rat, Human, Monkey
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1G4
• Specificity: Human NCAM / CD56
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 848

• Applicable Applications for anti-NCAM / CD56 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml); WB (1:200 - 1:4000); ; Optimal dilutions to be determined by the researcher.
• Immunogen: NCAM / CD56 antibody was raised against human recombinant protein fragment corresponding to amino acids 20-718 of human NCAM1 (NP_851996) produced in E Coli.
• Immunogen Species: NCAM/CD56 antibody was raised against Human
• Antigen Type: Recombinant protein
• Epitope: aa20-718
• Family/Subfamily: Immunoglobulin/ not assigned-Immunoglobulin
• Target: NCAM/CD56
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20°C. Avoid freeze-thaw cycles.

Related Product Information for anti-NCAM / CD56 antibody

NCAM1 Antibody, CD56 Antibody, CD56 antigen Antibody, MSK39 Antibody, NCAM-1 Antibody, N-CAM-1 Antibody, NCAM Antibody Description: NCAM / CD56 is a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.

NCBI and Uniprot Product Information

• NCBI GI #: 94420689
• NCBI GeneID: 4684
• NCBI Accession #: NP_000606.3
• NCBI GenBank Nucleotide #: NM_000615.6
• UniProt Accession #: P13591; P13592; P13593; Q05C58; Q15829; Q16180; Q16209; Q59FL7; Q86X47; Q96CJ3; A8K8T8
• NCBI Official Full Name: neural cell adhesion molecule 1 isoform 1
• NCBI Official Synonym Full Names: neural cell adhesion molecule 1
• NCBI Official Symbol: NCAM1
• NCBI Official Synonym Symbols: CD56; NCAM; MSK39
• NCBI Protein Information: neural cell adhesion molecule 1
• UniProt Protein Name: Neural cell adhesion molecule 1
• UniProt Gene Name: NCAM1
• UniProt Synonym Gene Names: NCAM; N-CAM-1; NCAM-1
• UniProt Entry Name: NCAM1_HUMAN

NCBI Description

This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]

Uniprot Description

NCAM1: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, GPI anchor; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: cell surface; cytoplasm; external side of plasma membrane; Golgi membrane; integral to membrane; membrane; plasma membrane

Molecular Function: identical protein binding; viral receptor activity

Biological Process: activation of MAPKK activity; axon guidance; cell adhesion; cytokine and chemokine mediated signaling pathway; entry of virus into host cell; epidermal growth factor receptor signaling pathway; extracellular matrix organization and biogenesis; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; nerve growth factor receptor signaling pathway; Ras protein signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway