Mouse Heat Shock 105kDa/110kDa Protein 1 (HSPH1) ELISA Kit | HSPH1 elisa kit

Mouse Heat Shock 105kDa/110kDa Protein 1 (HSPH1) ELISA Kit

Cat. Num. AAA2707633

• Gene Names: HSPH1; HSP105; HSP105A; HSP105B; NY-CO-25
• Reactivity: Mouse
• Synonyms: Heat Shock 105kDa/110kDa Protein 1 (HSPH1); Mouse Heat Shock 105kDa/110kDa Protein 1 (HSPH1) ELISA Kit; HSP105A; HSP105B; NY-CO-25; Heat shock protein 105 kDa; Heat shock 110 kDa protein; HSPH1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Heat Shock 105kDa/110kDa Protein 1 (HSPH1).; No significant cross-reactivity or interference between Heat Shock 105kDa/110kDa Protein 1 (HSPH1) and analogues was observed.
• Sequence Length: 858

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.60ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock 105kDa/110kDa Protein 1 (HSPH1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock 105kDa/110kDa Protein 1 (HSPH1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HSPH1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 105kDa/110kDa Protein 1 (HSPH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 105kDa/110kDa Protein 1 (HSPH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 105kDa/110kDa Protein 1 (HSPH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 105kDa/110kDa Protein 1 (HSPH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 42544159
• NCBI GeneID: 10808
• NCBI GenBank Nucleotide #: NP_006635.2
• UniProt Accession #: Q92598
• NCBI Official Full Name: heat shock protein 105 kDa isoform 1
• NCBI Official Synonym Full Names: heat shock protein family H (Hsp110) member 1
• NCBI Official Symbol: HSPH1
• NCBI Official Synonym Symbols: HSP105; HSP105A; HSP105B; NY-CO-25
• NCBI Protein Information: heat shock protein 105 kDa
• UniProt Protein Name: Heat shock protein 105 kDa
• Protein Family: Heat shock protein
• UniProt Gene Name: HSPH1
• UniProt Synonym Gene Names: HSP105; HSP110; KIAA0201
• UniProt Entry Name: HS105_HUMAN

NCBI Description

This gene encodes a member of the heat shock protein 70 family of proteins. The encoded protein functions as a nucleotide exchange factor for the molecular chaperone heat shock cognate 71 kDa protein (Hsc70). In addition, this protein plays a distinct but related role as a holdase that inhibits the aggregation of misfolded proteins, including the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Elevated expression of this protein has been observed in numerous human cancers. [provided by RefSeq, Mar 2017]

Uniprot Description

HSP105: a molecular chaperone of the heat shock protein 70 family. Has ATPase activity. Expressed at especially high levels in mammalian brain and has been shown to suppress apoptosis in neuronal cells and to prevent the aggregation of proteins following heat shock. Known to interact with a variety of proteins including alpha-tubulin and the androgen receptor. Two alternatively spliced isoforms have been described.

Protein type: Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 13q12.3

Cellular Component: nucleoplasm; microtubule; cytoplasm; extracellular region; cytosol

Molecular Function: protein binding; ATP binding; alpha-tubulin binding

Biological Process: receptor-mediated endocytosis; positive regulation of NK T cell activation; chaperone cofactor-dependent protein folding; positive regulation of MHC class I biosynthetic process; response to unfolded protein