Mouse Nav1.8 Monoclonal Antibody | anti-Scn10a antibody

Nav1.8 Antibody

Cat. Num. AAA800264

• Gene Names: Scn10a; PN3; Na(V)1.8
• Reactivity: Human, Mouse, Rat, Monkey
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein G Purified
• Synonyms: Nav1.8; Monoclonal Antibody; Nav1.8 Antibody; PN3; Na(V)1.8; Scn10a; anti-Scn10a antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Monkey
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: S134-12
• Specificity: Detects ~220kDa. No cross reactivity against other Nav channels.
• Purity/Purification: Protein G Purified
• Form/Format: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for anti-Scn10a antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Antibody Microarray (AM)
• Application Notes: WB (1:1000), IHC (1:1000), ICC/IF (1:100); Optimal dilutions for assays should be determined by the user.
• Certificate of Analysis: 1 ug/mL of MBS800264 was sufficient for detection of Nav1.8 in 10ug of COS cell lysate transiently expressing Nav1.8 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Immunogen: Fusion protein amino acids 1724-1956 of rat Nav1.8.
• Preparation and Storage: Ships on blue ice. Store at -20°C; 1 year+. Avoid freeze/thaw cycles.

Related Product Information for anti-Scn10a antibody

Nav1.8 is a voltage-gated sodium channel and plays a critical role in the generation and conduction of action potentials and is thus important for electrical signaling by most excitable cells. Therapeutically, the association of pain insensitivity with the loss of function of a certain sodium channel may have implications. Since Nav1.8 is not present in cardiac muscle or neurons in the central nervous system, blockers of Nav1.8 will not have direct action on these cells and thus can have less side effects than current pain medications. By performing more studies, there is a possibility to develop a new generation of drugs that can reduce the pain intensity in animals (3).

Product Categories/Family for anti-Scn10a antibody

Ion Channels; Neuroscience

References

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Dray A. (2008) Br. J. Anaesth. 101(1): 48-58. 4. Dray A., Read S.J (2007) Arthritis Res. Ther. 9(3): 212. 5. Samuels M.E., teMorshe R.H., Lynch M.E., Drenth J.P. (2008) Mol Pain. 4: 21.

NCBI and Uniprot Product Information

• NCBI GI #: 8394242
• NCBI GeneID: 29571
• NCBI Accession #: NP_058943.1
• NCBI GenBank Nucleotide #: NM_017247.1
• UniProt Accession #: Q62968; Q63554; Q6EWG6
• NCBI Official Full Name: sodium channel protein type 10 subunit alpha
• NCBI Official Synonym Full Names: sodium channel, voltage-gated, type X, alpha subunit
• NCBI Official Symbol: Scn10a
• NCBI Official Synonym Symbols: PN3; Na(V)1.8
• NCBI Protein Information: sodium channel protein type 10 subunit alpha; sensory neuron sodium channel; peripheral nerve sodium channel 3; sodium channel type X alpha polypeptide; sodium channel protein type X subunit alpha; voltage-gated sodium channel subunit alpha Nav1.8; sodium channel voltage-gated type X alpha polypeptide; sodium channel, voltage-gated, type 10, alpha polypeptide
• UniProt Protein Name: Sodium channel protein type 10 subunit alpha
• Protein Family: Sodium channel protein
• UniProt Gene Name: Scn10a
• UniProt Synonym Gene Names: Sns; PN3
• UniProt Entry Name: SCNAA_RAT

NCBI Description

voltage-gated sodium channel that is resistant to tetrodotoxin [RGD, Feb 2006]

Uniprot Description

SCN10A: This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant sodium channel isoform. Its electrophysiological properties vary depending on the type of the associated beta subunits (in vitro). Plays a role in neuropathic pain mechanisms. Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.8/SCN10A subfamily.

Protein type: Membrane protein, multi-pass; Channel, sodium; Membrane protein, integral