Rabbit anti-Human PIP5K1A Polyclonal Antibody | anti-PIP5K1A antibody

PIP5K1A, NT (Phosphatidylinositol-4-phosphate 5-kinase Type-1 alpha, PtdIns(4)P-5-Kinase alpha, PIP5KI-alpha, Phosphatidylinositol-4-phosphate 5-kinase Type I alpha, 68kD Type I Phosphatidylinositol-4-phosphate 5-kinase alpha, PIP5KIalpha) (Biotin)

Cat. Num. AAA6492992

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PIP5K1A; Polyclonal Antibody; NT (Phosphatidylinositol-4-phosphate 5-kinase Type-1 alpha; PtdIns(4)P-5-Kinase alpha; PIP5KI-alpha; Phosphatidylinositol-4-phosphate 5-kinase Type I alpha; 68kD Type I Phosphatidylinositol-4-phosphate 5-kinase alpha; PIP5KIalpha) (Biotin); anti-PIP5K1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PIP5K1A.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 549

• Applicable Applications for anti-PIP5K1A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human PIP5K1A (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PIP5K1A (arrow) using rabbit polyclonal hPIP5K1A-R34. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIP5K1A gene.)

Western Blot (WB)

(Western blot analysis of anti-PIP5K1A Pab in HeLa cell lysate. PIP5K1A (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Product Categories/Family for anti-PIP5K1A antibody

Antibodies; Protein Kinases

References

1. Doughman, R.L., et al., J. Biol. Chem. 278(25):23036-23045 (2003). 2. Loijens, J.C., et al., J. Biol. Chem. 271(51):32937-32943 (1996). 3. Xie, Y., et al., Cytogenet. Cell Genet. 88(3-4), 197-199 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 4505815
• NCBI GeneID: 8394
• NCBI Accession #: NP_003548
• NCBI GenBank Nucleotide #: NP_003548.1
• UniProt Accession #: Q99755
• Molecular Weight: 62.633
• NCBI Official Full Name: phosphatidylinositol 4-phosphate 5-kinase type-1 alpha isoform 2
• NCBI Official Synonym Full Names: phosphatidylinositol-4-phosphate 5-kinase type 1 alpha
• NCBI Official Symbol: PIP5K1A
• NCBI Protein Information: phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
• UniProt Protein Name: Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
• Protein Family: Phosphatidylinositol 4-phosphate 5-kinase
• UniProt Gene Name: PIP5K1A
• UniProt Synonym Gene Names: PIP5K1-alpha; PtdIns(4)P-5-kinase 1 alpha; PIP5KIalpha
• UniProt Entry Name: PI51A_HUMAN