Rat Myelin Basic Protein, aa82-87 Monoclonal Antibody | anti-MBP antibody

Myelin Basic Protein, aa82-87 (MBP)

Cat. Num. AAA6005719

• Reactivity: Bovine, Chicken, Guinea Pig, Human, Mammal, Mouse, Rabbit, Rat, Sheep
• Applications: ELISA, Immunohistochemistry, immunoassay, Radioimmunoassay
• Purity: Supernatant; Tissue culture supernatant.
• Synonyms: Myelin Basic Protein; aa82-87; Monoclonal Antibody; aa82-87 (MBP); Anti -Myelin Basic Protein; anti-MBP antibody

• Host: Rat
• Reactivity: Bovine, Chicken, Guinea Pig, Human, Mammal, Mouse, Rabbit, Rat, Sheep
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 3H2750
• Specificity: Reacts with myelin basic protein from a wide range of species. The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87. Mapped by Geyson method to DENPW method. Clone 3H2750 has been reported as being suitable for use in Western blotting.
• Purity/Purification: Supernatant; Tissue culture supernatant.
• Form/Format: Supplied as a liquid with 0.1% sodium azide.

• Applicable Applications for anti-MBP antibody: ELISA (EL/EIA), Immunohistochemistry (IHC), Radioimmunoassay (RIA)
• Application Notes: Suitable for use in Immunohistochemistry, ELISA and RIA.; Dilution: Immunohistochemistry: Frozen
• Immunogen: Bovine MBP.
• Fusion Partners: Spleen cells from an immunized outbred rat were fused with cells of the mouse NSO myeloma cell line.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-MBP antibody

Antibodies; Abs to Myelin

NCBI and Uniprot Product Information

• NCBI GI #: 1162922
• NCBI GeneID: 4155
• UniProt Accession #: P02686; Q15337; Q15338; Q15339; Q15340; A4FU54; A6NI84; A8MY86; A8MYL4; B3KY66; B7ZKS2; B7ZKS4
• Molecular Weight: 33,117 Da
• NCBI Official Full Name: myelin basic protein
• NCBI Official Synonym Full Names: myelin basic protein
• NCBI Official Symbol: MBP
• NCBI Protein Information: Golli-MBP; myelin basic protein; myelin A1 protein; myelin membrane encephalitogenic protein
• UniProt Protein Name: Myelin basic protein
• Protein Family: Myelin basic protein
• UniProt Gene Name: MBP
• UniProt Synonym Gene Names: MBP
• UniProt Entry Name: MBP_HUMAN

NCBI Description

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]

Uniprot Description

MBP: The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. Homodimer. Isoform 3 exists as a homodimer. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. Belongs to the myelin basic protein family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 18q23

Cellular Component: internode region of axon; compact myelin; cell soma; plasma membrane; nucleus

Molecular Function: protein binding; structural constituent of myelin sheath; protease binding

Biological Process: response to mercury ion; myelination; synaptic transmission; substantia nigra development; central nervous system development; response to toxin; immune response; negative regulation of axonogenesis; axon ensheathment; response to progesterone stimulus; aging