Mouse anti-Human E-Selectin Monoclonal Antibody | anti-SELE antibody

Mouse Anti-Human E-Selectin

Cat. Num. AAA690867

• Gene Names: SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein G affinity chromatography
• Synonyms: E-Selectin; Monoclonal Antibody; Mouse Anti-Human E-Selectin; Anti-human E-Selectin; anti-SELE antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2
• Clone Number: 9E7
• Specificity: This antibody recognizes human E-selectin in western blot. Reactivity to ESelectin of other species is not tested. No cross reactivity to ICAM, and VCAM.
• Purity/Purification: Protein G affinity chromatography
• Form/Format: Lyophilized
• Sequence Length: 610

• Applicable Applications for anti-SELE antibody: Western Blot (WB), Immunohistochemistry (IHC) - Paraffin
• Application Notes: 1. Western blotting: Yes (1:500-2000) 2. Immunohistochemistry: Yes (1:50-200) (Paraffin sections)
• Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified human recombinant E-selectin protein. The IgG2 fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.
• Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.
• Preparation and Storage: Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

Related Product Information for anti-SELE antibody

E-Selectin (Endothelial Leukocyte Adhesion Molecule1, ELAM1, CD62E), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL 1beta and TNFalpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and mouse E-Selectin proteins share 81% amino acid similarity. E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

NCBI and Uniprot Product Information

• NCBI GI #: 187960042
• NCBI GeneID: 6401
• NCBI Accession #: NP_000441.2
• NCBI GenBank Nucleotide #: NM_000450.2
• UniProt Accession #: P16581; P16111; A2RRD6
• Molecular Weight: 66,655 Da
• NCBI Official Full Name: E-selectin
• NCBI Official Synonym Full Names: selectin E
• NCBI Official Symbol: SELE
• NCBI Official Synonym Symbols: ELAM; ESEL; CD62E; ELAM1; LECAM2
• NCBI Protein Information: E-selectin; ELAM-1; endothelial adhesion molecule 1; CD62 antigen-like family member E; endothelial leukocyte adhesion molecule 1; leukocyte endothelial cell adhesion molecule 2; leukocyte-endothelial cell adhesion molecule 2
• UniProt Protein Name: E-selectin
• Protein Family: E-selectin
• UniProt Gene Name: SELE
• UniProt Synonym Gene Names: ELAM1; ELAM-1; LECAM2
• UniProt Entry Name: LYAM2_HUMAN

NCBI Description

The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. Ref.1

Subunit structure: Interacts with PSGL1/SELPLG and PODXL2 through the sialyl Lewis X epitope. PSGL1 sulfation appears not to be required for this interaction. Ref.9 Ref.12

Subcellular location: Membrane; Single-pass type I membrane protein.

Polymorphism: A polymorphism in position 149 is associated with a higher risk of coronary artery disease (CAD). A significantly higher mutation frequency (Arg-149) is observed in patients with angiographically proven severe atherosclerosis compared with an unselected population (Ser-149).

Sequence similarities: Belongs to the selectin/LECAM family.Contains 1 C-type lectin domain.Contains 1 EGF-like domain.Contains 6 Sushi (CCP/SCR) domains.