Rabbit CKB Polyclonal Antibody | anti-CKB antibody

CKB Polyclonal Antibody

Cat. Num. AAA3012483

• Gene Names: CKB; BCK; B-CK; CKBB; CPK-B; HEL-211; HEL-S-29
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: CKB; Polyclonal Antibody; CKB Polyclonal Antibody; B-CK; CPK-B; CKBB; B CK; BB-CK; BCK; Brain creatine kinase; Ckb; Creatine kinase B; Creatine kinase B chain; Creatine kinase B type; Creatine kinase B-type; Creatine Kinase BB Isoenzyme; Creatine kinase brain; Creatine kinase brain type; Creatine phosphokinase BB; Epididymis luminal protein 211; Epididymis secretory protein Li 29; HEL 211; HEL S 29; KCRB; anti-CKB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CKB polyclonal antibody detects endogenous levels of CKB protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 381

• Applicable Applications for anti-CKB antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:2,000; ICC: 1:50-1:200; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: Recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Creatine kinase B type on different lysates using anti-Creatine kinase B type antibody at 1/500 dilution. Positive control:Lane 1: Mouse brain Lane 2: Mouse brainLane 3: Rat brain Lane 4: 293)

Immunocytochemistry (ICC)

(ICC staining Creatine kinase B type in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-CKB antibody

Creatine kinases (CKs) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. Creatine kinases provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems including muscle, electrocytes, retina photoreceptor cells, brain cells, kidney, salt glands, myometrium, placenta, pancreas, thymus, thyroid, intestinal epithelial cells, endothelial cells, cartilage and bone cells, macrophages, blood platelets, and tumor and cancer cells. Human cytoplasmic creatine kinase-B, also designated CK-B and BCK, is a 381 amino acid, brain tissue specific isoform of creatine kinase. Human cytoplasmic creatine kinase-muscle (CK-M, MCK) is a muscle tissue-specific isoform of creatine kinase. Human cytoplasmic creatine kinase-Mi (Mi-CK, MtCK) is a 416 amino acid mitochondrial-specific isoform of creatine kinase. Cytosolic creatine kinases are important in the energetic regulation of Ca2+-pumps and in the maintenance of Ca2+-homeostasis.

NCBI and Uniprot Product Information

• NCBI GI #: 125294
• NCBI GeneID: 1152
• UniProt Accession #: P12277; Q04447; P07335
• Molecular Weight: 43kDa
• NCBI Official Full Name: Creatine kinase B-type
• NCBI Official Synonym Full Names: creatine kinase B
• NCBI Official Symbol: CKB
• NCBI Official Synonym Symbols: BCK; B-CK; CKBB; CPK-B; HEL-211; HEL-S-29
• NCBI Protein Information: creatine kinase B-type
• UniProt Protein Name: Creatine kinase B-type
• Protein Family: Creatine kinase
• UniProt Gene Name: CKB
• UniProt Synonym Gene Names: CKBB
• UniProt Entry Name: KCRB_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

CKB: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: Amino Acid Metabolism - arginine and proline; EC 2.7.3.2; Kinase, other

Chromosomal Location of Human Ortholog: 14q32

Cellular Component: mitochondrion; cytosol

Molecular Function: protein binding; creatine kinase activity; ATP binding

Biological Process: substantia nigra development; phosphorylation; creatine metabolic process; cellular chloride ion homeostasis