Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-MS4A4A antibody 3F2 (MBS488677) Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 3F2 (MBS488677) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488677, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Mouse anti-Human MS4A4A Monoclonal Antibody | anti-MS4A4A antibody
Anti-MS4A4A [3F2]
Gene Names
MS4A4A; MS4A4; MS4A7; 4SPAN1; CD20L1; CD20-L1; HDCME31P
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
Purified antibody. Protein A affinity purified
Synonyms
MS4A4A; Monoclonal Antibody; Anti-MS4A4A [3F2]; Four-span transmembrane protein 1; human membrane-spanning 4-domains subfamily A member 4A; CD20 antigen-like 1; (MS4A4A)-GFP; MS4A4; 4SPAN1; MS4A; anti-MS4A4A antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1, kappa
Clone Number
3F2
Specificity
This antibody is specific for human extracellular MS4A4A. The 3F2 and 4H2 (Ab01373) mAb clones completely cross-block one another indicating that they might bind to the same epitope or proximal epitopes (Sanyal et al., 2017; pmid: 28303902). MS4A (membrane-spanning 4-domain family, subfamily A) is a large family of proteins that includes at least 26 members in mouse and humans. Flanked by amino- and carboxyl- cytoplasmic regions, MS4A family members contain four highly conserved transmembrane domains connected by short extracellular and cytoplasmic loops. MS4A4A is likely to be a component of oligomeric cell surface complexes involved in signal transduction in diverse cell lineages.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-MS4A4A antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
3F2 is recommended for the detection and analysis of expression of MS4A4A. Importantly, MS4A4A has been shown to be a marker for M2 macrophages and plasma cells and to be overexpressed in multiple myelomas, suggesting a potential role in targeting alternatively activated macrophages such as tumor-associated macrophages, and in the treatment of multiple myeloma and mantle cell lymphoma (Sanyal et al., 2017; pmid: 28303902). The antibody 3F2 can be successfully used for the detection of MS4A4A-expressing cells via flow cytometry (Sanyal et al., 2017; pmid: 28303902). Finally, specificity of this antibody was additionally confirmed via immunoprecipitation of lysates of BJAB MS4A4A-HA cells (Sanyal et al., 2017; pmid: 28303902).
PMID
28303902
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-MS4A4A antibody 3F2 (MBS488677) Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 3F2 (MBS488677) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488677, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed K562 cells with anti-MS4A4A antibody 3F2 (MBS488677) Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 3F2 (MBS488677) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488677, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-MS4A4A antibody
Recombinant monoclonal antibody to MS4A4A. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3F2.
This antibody was generated by immunizing C57BL/6 mice against mouse cell line 300-19 expressing transfected human membrane-spanning 4-domains subfamily A member 4A (MS4A4A)-GFP.
This antibody was generated by immunizing C57BL/6 mice against mouse cell line 300-19 expressing transfected human membrane-spanning 4-domains subfamily A member 4A (MS4A4A)-GFP.
Product Categories/Family for anti-MS4A4A antibody
References
Sanyal et al. MS4A4A: a novel cell surface marker for M2 macrophages and plasma cells. Immunol Cell Biol. 2017 Aug;95(7):611-619. doi: 10.1038/icb.2017.18. Epub 2017 Mar 17.
Note on publication: This article describes the generation and characterization of the antibody 3F2.
Note on publication: This article describes the generation and characterization of the antibody 3F2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,441 Da
NCBI Official Full Name
membrane-spanning 4-domains subfamily A member 4A isoform 3
NCBI Official Synonym Full Names
membrane-spanning 4-domains, subfamily A, member 4A
NCBI Official Symbol
MS4A4A
NCBI Official Synonym Symbols
MS4A4; MS4A7; 4SPAN1; CD20L1; CD20-L1; HDCME31P
NCBI Protein Information
membrane-spanning 4-domains subfamily A member 4A; CD20 antigen-like 1; four-span transmembrane protein 1; Fc epsilon receptor beta subunit homolog; membrane-spanning 4-domains, subfamily A, member 4
UniProt Protein Name
Membrane-spanning 4-domains subfamily A member 4A
Protein Family
UniProt Gene Name
MS4A4A
UniProt Synonym Gene Names
4SPAN1; CD20L1; MS4A4; HDCME31P
UniProt Entry Name
M4A4A_HUMAN
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Product Notes
The MS4A4A ms4a4a (Catalog #AAA488676) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-MS4A4A [3F2] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MS4A4A can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunoprecipitation (IP). 3F2 is recommended for the detection and analysis of expression of MS4A4A. Importantly, MS4A4A has been shown to be a marker for M2 macrophages and plasma cells and to be overexpressed in multiple myelomas, suggesting a potential role in targeting alternatively activated macrophages such as tumor-associated macrophages, and in the treatment of multiple myeloma and mantle cell lymphoma (Sanyal et al., 2017; pmid: 28303902). The antibody 3F2 can be successfully used for the detection of MS4A4A-expressing cells via flow cytometry (Sanyal et al., 2017; pmid: 28303902). Finally, specificity of this antibody was additionally confirmed via immunoprecipitation of lysates of BJAB MS4A4A-HA cells (Sanyal et al., 2017; pmid: 28303902). Researchers should empirically determine the suitability of the MS4A4A ms4a4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MS4A4A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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