Rat anti-Mouse Monocytes/Macrophages Monoclonal Antibody
Monocytes/Macrophages, Mouse, mAb ER-HR3
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Synonyms
Monocytes/Macrophages; Monoclonal Antibody; Mouse; mAb ER-HR3; anti-Monocytes/Macrophages antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2c
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Applicable Applications for anti-Monocytes/Macrophages antibody
F, Flow Cytometry (FC/FACS), P
Application Notes
Application Use: For flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-Monocytes/Macrophages antibody
Product Description: The monoclonal antibody ER-HR3 is reactive to the majority of mouse monocytes and a subset of mature resident macrophages, especially those located in hemopoietic organs. Whereas all other leukocytes are ER-HR3 negative, up to 70% of circulating monocytes stain positive with this monoclonal antibody. Furthermore, about 30% of bone marrow cells express the antigen. Other ER-HR3 positive macrophages can be found in the splenic red pulp, in the mesenteric lymphoid paracortex, interfollicular areas of Peyer's patches and bone marrow. Few ER-HR3 positive cells are observed in the thymic cortex and the connective tissues of the gastro-intestinal tract, the dermis and the renal medulla. Epidermal Langerhans cells also express the antigen. The monoclonal antibody ER-HR3 can be used to identify and localize a very distinct mature tissue macrophage subpopulation found in various mouse organs and its distribution pattern is unique compared to that from other macrophage-specific antibodies. The antibody is especially suitable for ontogenic studies because ER-HR3 positive macrophages are closely related to hemopoietic islands, especially at erythropoietic sites.
References
1. de Jong, J et al; A monoclonal antibody (ER-HR3) against murine macrophages. I. Ontogeny, distribution and enzyme histochemical characterization of ER-HR3-positive cells. Cell Tissue Res 1994, 275: 567 2. de Jong, J et al; A monoclonal antibody (ER-HR3) against murine macrophages. II. Biochemical and functional aspects of the ER-HR3 antigen. Cell Tissue Res 1994, 275: 577
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Product Notes
The Monocytes/Macrophages (Catalog #AAA584253) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Monocytes/Macrophages, Mouse, mAb ER-HR3 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Monocytes/Macrophages can be used in a range of immunoassay formats including, but not limited to, F, Flow Cytometry (FC/FACS), P. Application Use: For flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the Monocytes/Macrophages for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Monocytes/Macrophages, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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