Immunohistochemistry
(IHC-F: Immunohistochemistry on frozen C3H/HeN ear section stained with Clone ER-MP23 (5ug/ml))
Rat anti-Mouse MGL Monoclonal Antibody | anti-MGL antibody
MGL, Mouse, mAb ER-MP23
Reactivity
Mouse
Applications
Immunohistochemistry, Immunohistochemistry, Flow Cytometry
Purity
Protein G
Synonyms
MGL; Monoclonal Antibody; Mouse; mAb ER-MP23; anti-MGL antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a
Purity/Purification
Protein G
Form/Format
0.2um filtered in PBS + 0.1% BSA
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Concentration
100ug/ml (varies by lot)
Applicable Applications for anti-MGL antibody
IHC-F, IHC-P, FC, FS
Application Notes
Application Use: For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For blocking studies in vitro, dilutions have to be made according to the amounts of MGL to be inactivated. The antigen is glutaraldehyde (0.05%) resistant.
Endotoxin
2.95 EU/mg
Immunogen
Mouse macrophage cell lines
Aliases
Macrophage galactose-specific lectin, CD301, dendritic cell-asialoglycoprotein receptor, DC-ASGPR.
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Immunohistochemistry
(IHC-F: Immunohistochemistry on frozen C3H/HeN ear section stained with Clone ER-MP23 (5ug/ml))
Immunohistochemistry
(IHC-F: Immunohistochemistry on frozen C3H/HeN ear section stained with Clone ER-MP23 (5ug/ml))
Related Product Information for anti-MGL antibody
Product Description: The monoclonal antibody ER-MP23 specifically reacts with the macrophage galactose-specific lectin (MGL), a 38 kDa single chain surface glycoprotein. MGL is found on murine mature macrophages in the connective tissue neighboring epithelia of e.g. salivary glands, capsule of lymph nodes, thymus and various other organs. MGL is present on the surface of connective tissue macrophages and their precursor cells in bone marrow. Also, MGL is expressed in macrophage cell lines (e.g. J774-1.6, RAW309Cr.1 and WR19M.1). Expression levels of MGL are increased in mature macrophages. The antigen is co expressed with the antigen recognized by monoclonal antibody BM8 (HM1066). The monoclonal antibody ER-MP23 has been raised after immunization of rats with mouse macrophage cell lines. Blocking studies demonstrated that the monoclonal antibody ER-MP23 is able to block mouse MGL.
References
1. Jansen, A et al; Immunohistochemical characterization of monocytes-macrophages and dendritic cells involved in the initiation of the insulits and beta-cell destruction in NOD mice. Diabetes 1994, 43: 667 2. Rosmalen, J et al; Islet abnormalities associated with an early influx of dendritic cells and macrophages in NOD and NODscid mice. Lab Invest 2000, 80: 769
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Product Notes
The MGL (Catalog #AAA584265) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The MGL, Mouse, mAb ER-MP23 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MGL can be used in a range of immunoassay formats including, but not limited to, IHC-F, IHC-P, FC, FS. Application Use: For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For blocking studies in vitro, dilutions have to be made according to the amounts of MGL to be inactivated. The antigen is glutaraldehyde (0.05%) resistant. Researchers should empirically determine the suitability of the MGL for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MGL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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