Mouse anti-Human AKT1S1 Monoclonal Antibody | anti-AKT1S1 antibody

AKT1S1 (PRAS40, Proline-rich AKT1 substrate 1, 40kD proline-rich AKT substrate) (Biotin)

Cat. Num. AAA6121124

• Gene Names: AKT1S1; Lobe; PRAS40
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: AKT1S1; Monoclonal Antibody; AKT1S1 (PRAS40; Proline-rich AKT1 substrate 1; 40kD proline-rich AKT substrate) (Biotin); PRAS40; 40kD proline-rich AKT substrate; anti-AKT1S1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 449CT13.4.2
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-AKT1S1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:1,000; WB: 1:100; Applications are based on unconjugated antibody.
• Immunogen: Purified His-tagged AKT1S1 protein (fragment).
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(AKT1S1 Antibody western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the AKT1S1 antibody detected the AKT1S1 protein (arrow).)

Related Product Information for anti-AKT1S1 antibody

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]). [supplied by OMIM].

Product Categories/Family for anti-AKT1S1 antibody

Antibodies; 14-3-3

References

Wang, L., et al. J. Biol. Chem. 283(23):15619-15627(2008) Fonseca, B.D., et al. Biochem. J. 411(1):141-149(2008) Fonseca, B.D., et al. J. Biol. Chem. 282(34):24514-24524(2007) Wang, L., et al. J. Biol. Chem. 282(27):20036-20044(2007) Sancak, Y., et al. Mol. Cell 25(6):903-915(2007)

NCBI and Uniprot Product Information

• NCBI GI #: 148806906
• NCBI GeneID: 84335
• NCBI Accession #: NP_001092102.1
• NCBI GenBank Nucleotide #: NM_001098632.2
• UniProt Accession #: Q96B36; Q96BI4; Q96IK7; Q96NG2; Q9BWR5; A8MTQ1; B2RE93
• Molecular Weight: 27,383 Da
• NCBI Official Full Name: proline-rich AKT1 substrate 1 isoform b
• NCBI Official Synonym Full Names: AKT1 substrate 1 (proline-rich)
• NCBI Official Symbol: AKT1S1
• NCBI Official Synonym Symbols: Lobe; PRAS40
• NCBI Protein Information: proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate
• UniProt Protein Name: Proline-rich AKT1 substrate 1
• Protein Family: Proline-rich AKT1 substrate
• UniProt Gene Name: AKT1S1
• UniProt Synonym Gene Names: PRAS40
• UniProt Entry Name: AKTS1_HUMAN

NCBI Description

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008]

Uniprot Description

Function: Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection. Ref.6 Ref.8 Ref.9

Subunit structure: Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Interacts directly with RPTOR. The phosphorylated form interacts with 14-3-3 proteins. Ref.5 Ref.8 Ref.9

Subcellular location: Cytoplasm › cytosol

By similarity. Note: Found in the cytosolic fraction of the brain

By similarity.

Tissue specificity: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293). Ref.5 Ref.6

Post-translational modification: Phosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1. Ref.5

Sequence caution: The sequence AAH00031.2 differs from that shown. Reason: Erroneous initiation.