Western Blot (WB)
(Western Blot analysis of HeLa cells using MMS19 Monoclonal Antibody at dilution of 1:2000)
Mouse MMS19 Monoclonal Antibody | anti-MMS19 antibody
MMS19 Monoclonal Antibody
Gene Names
MMS19; MET18; MMS19L; hMMS19
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Protein A purification
Synonyms
MMS19; Monoclonal Antibody; MMS19 Monoclonal Antibody; hMMS19; homolog of yeast MMS19; MET18; MET18 homolog; MET18MMS19 nucleotide excision repair homolog (S. cerevisiae); mms19; MMS19 nucleotide excision repair protein homolog; MMS19 nucleotide excision repair; S. cerevisiae; homolog of; MMS19-like (MET18 homolog; S. cerevisiae); MMS19-like protein; MMS19L; anti-MMS19 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
12
Purity/Purification
Protein A purification
Concentration
1mg/mL (varies by lot)
Sequence Length
987
Applicable Applications for anti-MMS19 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
IHC: 1:20-1:200
IF: 1:20-1:200
Immunogen
Fusion protein of MMS19
Buffer
PBS with 0.02% sodium azide, 50% glycerol, PH7.3
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western Blot analysis of HeLa cells using MMS19 Monoclonal Antibody at dilution of 1:2000)
Western Blot (WB)
(Western Blot analysis of HeLa cells using MMS19 Monoclonal Antibody at dilution of 1:2000)
Related Product Information for anti-MMS19 antibody
MMS19 (MMS19 nucleotide excision repair homolog), also known as MET18, is a 1, 030 amino acid nuclear protein containing seven HEAT repeats that belongs to the MET18/MMS19 family. Via its interactions with TFIIH p80 and TFIIH p89 helicases, MMS19 plays a role in nucleotide excision repair (NER) and RNA polymerase II (Pol II) transcription. MMS19 may also function as a transcriptional coactivator of estrogen receptor. While ubiquitously expressed, highest levels of MMS19 have been found in testis. At least five distinct MMS19 protein isoforms exist, which are produced by alternative splicing events.
Product Categories/Family for anti-MMS19 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated: 113kDa
Observed: 103kDa; 38kDa
Observed: 103kDa; 38kDa
NCBI Official Full Name
MMS19 nucleotide excision repair protein homolog isoform 2
NCBI Official Synonym Full Names
MMS19 homolog, cytosolic iron-sulfur assembly component
NCBI Official Symbol
MMS19
NCBI Official Synonym Symbols
MET18; MMS19L; hMMS19
NCBI Protein Information
MMS19 nucleotide excision repair protein homolog
UniProt Protein Name
MMS19 nucleotide excision repair protein homolog
Protein Family
UniProt Gene Name
MMS19
UniProt Synonym Gene Names
hMMS19
Uniprot Description
Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins such as ERCC2/XPD, FANCJ and RTEL1, thereby playing a key role in nucleotide excision repair (NER), homologous recombination-mediated double-strand break DNA repair, DNA replication and RNA polymerase II (POL II) transcription (PubMed:22678362, PubMed:22678361, PubMed:29225034). As part of the mitotic spindle-associated MMXD complex, plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD (PubMed:20797633). Indirectly acts as a transcriptional coactivator of estrogen receptor (ER), via its role in iron-sulfur insertion into some component of the TFIIH-machinery (PubMed:11279242).
Research Articles on MMS19
Similar Products
Product Notes
The MMS19 mms19 (Catalog #AAA2541987) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MMS19 Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MMS19 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:1000-1:10000 IHC: 1:20-1:200 IF: 1:20-1:200. Researchers should empirically determine the suitability of the MMS19 mms19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MMS19, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
