Monkey Basic Fibroblast growth factor receptor ELISA Kit | bFGFR elisa kit

Monkey Basic Fibroblast growth factor receptor ELISA Kit

Cat. Num. AAA740807

• Gene Names: FGF2; BFGF; FGFB; FGF-2; HBGF-2
• Reactivity: Monkey
• Synonyms: Basic Fibroblast growth factor receptor; Monkey Basic Fibroblast growth factor receptor ELISA Kit; bFGFR elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 2.5-50ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This bFGFR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey bFGFR. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for bFGFR elisa kit

Principle of the Assay: bFGFR ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for bFGFR. Standards or samples are then added to the microtiter plate wells and bFGFR if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of bFGFR present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for bFGFR are added to each well to "sandwich" the bFGFR immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain bFGFR and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The bFGFR concentration in each sample is interpolated from this standard curve.

Product Categories/Family for bFGFR elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 183087
• NCBI GeneID: 2247
• Molecular Weight: 21,203 Da
• NCBI Official Full Name: basic fibroblast growth factor, partial
• NCBI Official Synonym Full Names: fibroblast growth factor 2 (basic)
• NCBI Official Symbol: FGF2
• NCBI Official Synonym Symbols: BFGF; FGFB; FGF-2; HBGF-2
• NCBI Protein Information: fibroblast growth factor 2; prostatropin; heparin-binding growth factor 2; basic fibroblast growth factor bFGF
• UniProt Protein Name: Fibroblast growth factor 2
• UniProt Gene Name: FGF2
• UniProt Synonym Gene Names: FGFB; FGF-2; bFGF; HBGF-2
• UniProt Entry Name: FGF2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF2: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative initiation.

Protein type: Activator; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q26

Cellular Component: extracellular space; extracellular region; nucleus

Molecular Function: heparin binding; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; growth factor activity; cytokine activity; chemoattractant activity; fibroblast growth factor receptor binding

Biological Process: extracellular matrix organization and biogenesis; wound healing; nerve growth factor receptor signaling pathway; activation of MAPK activity; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; chemotaxis; signal transduction; hyaluronan catabolic process; growth factor dependent regulation of satellite cell proliferation; positive regulation of MAP kinase activity; positive chemotaxis; positive regulation of cell proliferation; embryonic morphogenesis; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; nervous system development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of cell fate specification; positive regulation of blood vessel endothelial cell migration; positive regulation of phosphoinositide 3-kinase activity; negative regulation of blood vessel endothelial cell migration; regulation of angiogenesis; organ morphogenesis; positive regulation of angiogenesis; cell migration during sprouting angiogenesis; ureteric bud branching; positive regulation of cell division; release of sequestered calcium ion into cytosol; Ras protein signal transduction; insulin receptor signaling pathway; phosphatidylinositol biosynthetic process; innate immune response; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; inositol phosphate biosynthetic process