Mouse anti-Human MLKL Monoclonal Antibody | anti-MLKL antibody

Phospho-MLKL (Ser358) Monoclonal Antibody

Cat. Num. AAA2548822

• Gene Names: MLKL; hMLKL
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A purification
• Synonyms: MLKL; Monoclonal Antibody; Phospho-MLKL (Ser358) Monoclonal Antibody; anti-MLKL antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 1D4
• Purity/Purification: Protein A purification
• Form/Format: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
• Sequence Length: 263

• Applicable Applications for anti-MLKL antibody: Immunohistochemistry Paraffin (IHC-p)
• Application Notes: IHC: 1:100-200
• Immunogen: Synthetic Peptide of Phospho-MLKL (Ser358)
• Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human colon carcinoma tissue with Phospho-MLKL (Ser358) Monoclonal Antibody at dilution of 1:200)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human skin tissue with Phospho-MLKL (Ser358) Monoclonal Antibody at dilution of 1:200)

Related Product Information for anti-MLKL antibody

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNFinduced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.MLKL (Mixed Lineage Kinase Domain Like Pseudokinase) is a Protein Coding gene. Among its related pathways are Apoptosis and Autophagy and CDK-mediated phosphorylation and removal of Cdc6. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity.

Product Categories/Family for anti-MLKL antibody

Acetyl/Phospho/Methyl Antibody; Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 215820622
• NCBI GenBank Nucleotide #: NM_001142497.2
• UniProt Accession #: Q8NB16; Q8N6V0; A6NCE4
• NCBI Official Full Name: mixed lineage kinase domain-like protein isoform 2
• NCBI Official Synonym Full Names: mixed lineage kinase domain like pseudokinase
• NCBI Official Symbol: MLKL
• NCBI Official Synonym Symbols: hMLKL
• NCBI Protein Information: mixed lineage kinase domain-like protein
• UniProt Protein Name: Mixed lineage kinase domain-like protein
• Protein Family: Mixed lineage kinase domain-like protein
• UniProt Gene Name: MLKL
• UniProt Synonym Gene Names: hMLKL

NCBI Description

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

MLKL: Belongs to the protein kinase superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; TKL-Unique family

Chromosomal Location of Human Ortholog: 16q23.1

Cellular Component: cytoplasm; cytosol; plasma membrane

Molecular Function: ATP binding; protein binding; protein complex binding; protein kinase activity; protein kinase binding; signal transducer activity

Biological Process: necroptotic process; protein amino acid phosphorylation