Rabbit anti-Human, Mouse MLKL Polyclonal Antibody | anti-MLKL antibody

MLKL Polyclonal Antibody

Cat. Num. AAA9133491

• Gene Names: MLKL; hMLKL
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: MLKL; Polyclonal Antibody; MLKL Polyclonal Antibody; hMLKL; anti-MLKL antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 263

• Applicable Applications for anti-MLKL antibody: Western Blot (WB)
• Application Notes: WB: 1:500 - 1:2000
• Immunogen: Recombinant protein of mouse MLKL
• Immunogen Species: Mouse
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Cellular Location: Cell membrane, Cytoplasm
• Positive Samples: HeLa, Mouse liver
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MLKL antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

Related Product Information for anti-MLKL antibody

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.

Product Categories/Family for anti-MLKL antibody

Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 215820622
• NCBI GeneID: 197259
• NCBI Accession #: NP_001135969.1
• NCBI GenBank Nucleotide #: NM_001142497.2
• UniProt Accession #: Q8NB16; Q8N6V0; A6NCE4
• Molecular Weight: Calculated: 53kDa; 54kDa; Observed: 50kDa
• NCBI Official Full Name: mixed lineage kinase domain-like protein isoform 2
• NCBI Official Synonym Full Names: mixed lineage kinase domain like pseudokinase
• NCBI Official Symbol: MLKL
• NCBI Official Synonym Symbols: hMLKL
• NCBI Protein Information: mixed lineage kinase domain-like protein
• UniProt Protein Name: Mixed lineage kinase domain-like protein
• Protein Family: Mixed lineage kinase domain-like protein
• UniProt Gene Name: MLKL
• UniProt Synonym Gene Names: hMLKL

NCBI Description

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.