Mouse anti-Human MLH1 Monoclonal Antibody | anti-MLH1 antibody

MLH1 Antibody

Cat. Num. AAA346017

• Gene Names: MLH1; FCC2; COCA2; HNPCC; hMLH1; HNPCC2
• Reactivity: Human
• Applications: Immunohistochemistry
• Synonyms: MLH1; Monoclonal Antibody; MLH1 Antibody; anti-MLH1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: IHC409
• Form/Format: Liquid
• Sequence Length: 34

• Applicable Applications for anti-MLH1 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Dilution Range: 1:100 - 1:200
• Buffer: Tris Buffer, ph7.4, 1% BSA, <0.1% NaN3
• IHC Positive Control: Colon, Colon Carcinoma
• IHC Suggested Protocol: 1. Tissues are fixed with 10% neutral buffered formalin, cut to ~4um, and placed on a positively charged glass slide.; 2. The prepared slide is baked for at least 30 minutes and at most 24 hours at 55-65 degree C.; 3. Pretreatment - Perform heat-induced epitope retrieval (HIER) at pH 6 for 10 to 30 minutes.; 4. Block – Block with peroxidase solution for 5 minutes at RT.; 5. Primary Ab – Dilute antibodies 1:100 – 1:200 and incubate for 10 - 30 minutes at RT.; 6. Secondary Ab - Incubate for 20 - 30 minutes at RT.; 7. Substrate - Incubate DAB or Fast Red for 5 - 10 minutes at RT.; 8. Counterstain - Counterstain with hematoxylin for 30 seconds to 5 minutes, rinse with distilled water and blueing solution for 30 seconds.; 9. Dehydrate and apply coverslip.; (Each researcher should optimize the protocol for their experimental model system.)
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.

Immunohistochemistry (IHC)

(IHC (paraffin) of human esophagus using MLH1 Antibody)

Related Product Information for anti-MLH1 antibody

MLH1 (MutL Homolog 1) is part of the mismatch-repair pathway, often associated with hereditary non-polyposis colorectal cancer. The protein is often deficient in endometrial and ovarian cancer patients. Anti-MLH1 antibodies can be optimized when paired along with other antibodies such as MSH6, MSH2, and PMS2.

NCBI and Uniprot Product Information

• NCBI GI #: 237930358
• NCBI GeneID: 4292
• Molecular Weight: 73,837 Da
• NCBI Official Full Name: MLH1, partial
• NCBI Official Synonym Full Names: mutL homolog 1
• NCBI Official Symbol: MLH1
• NCBI Official Synonym Symbols: FCC2; COCA2; HNPCC; hMLH1; HNPCC2
• NCBI Protein Information: DNA mismatch repair protein Mlh1
• UniProt Protein Name: DNA mismatch repair protein Mlh1
• Protein Family: Putative MutL protein
• UniProt Gene Name: MLH1
• UniProt Synonym Gene Names: COCA2

NCBI Description

The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). [provided by RefSeq, Aug 2017]

Uniprot Description

Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.