Chicken Interleukin 18 ELISA Kit | IL18 elisa kit

Chicken Interleukin 18 ELISA Kit

Cat. Num. AAA737017

• Gene Names: Il18; IL-18
• Reactivity: Chicken
• Synonyms: Interleukin 18; Chicken Interleukin 18 ELISA Kit; IL18 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 1.0 pg/mL
• Intended Uses: This IL18 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken IL18. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL18 elisa kit

Principle of the Assay: IL18 ELISAkit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL18. Standards or samples are then added to the microtiter plate wells and IL18 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL18 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for IL18 are added to each well to "sandwich" the IL18 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL18 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL18 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for IL18 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 9506805
• NCBI GeneID: 29197
• NCBI Accession #: NP_062038.1
• NCBI GenBank Nucleotide #: NM_019165.1
• UniProt Accession #: P97636; O88749; P97637; Q541E6
• Molecular Weight: 22,303 Da
• NCBI Official Full Name: interleukin-18
• NCBI Official Synonym Full Names: interleukin 18
• NCBI Official Symbol: Il18
• NCBI Official Synonym Symbols: IL-18
• NCBI Protein Information: interleukin-18; IL-1 gamma; interleukin-1 gamma; IFN-gamma-inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor
• UniProt Protein Name: Interleukin-18
• Protein Family: Interleukin
• UniProt Gene Name: Il18
• UniProt Synonym Gene Names: Igif; IL-18; IFN-gamma-inducing factor; IL-1 gamma
• UniProt Entry Name: IL18_RAT

NCBI Description

a pro-inflammatory cytokine; acts as a modulator of immune functions [RGD, Feb 2006]

Uniprot Description

Function: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.

Subcellular location: Secreted.

Sequence similarities: Belongs to the IL-1 family.