Rabbit MLH1 Polyclonal Antibody | anti-MLH1 antibody

MLH1 Antibody

Cat. Num. AAA9409984

• Gene Names: MLH1; FCC2; COCA2; HNPCC; hMLH1; HNPCC2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: MLH1; Polyclonal Antibody; MLH1 Antibody; COCA2; FCC2; HNPCC; HNPCC2; anti-MLH1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total MLH1 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 756

• Applicable Applications for anti-MLH1 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500 - 1:2000
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human MLH1.
• Target Name: MLH1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MLH1 antibody.)

Related Product Information for anti-MLH1 antibody

Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MLH1 is the human homologue of the E. coli MMR gene mutL. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-a), PMS2 (MutL-b) or MLH3 (MutL-g). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases and DNA ligase (reviewed in 1). Inactivation of the MLH1 gene causes genome instability and predisposition to cancer (2-5). The MLH1 gene is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) (6). MLH1 also plays a role in meiotic recombination (7).

Product Categories/Family for anti-MLH1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4557757
• NCBI GeneID: 4292
• NCBI Accession #: NP_000240.1
• NCBI GenBank Nucleotide #: NM_000249.3
• UniProt Accession #: P40692; B4DI13; B4DQ11; E9PCU2
• Molecular Weight: 73,837 Da
• NCBI Official Full Name: DNA mismatch repair protein Mlh1 isoform 1
• NCBI Official Synonym Full Names: mutL homolog 1
• NCBI Official Symbol: MLH1
• NCBI Official Synonym Symbols: FCC2; COCA2; HNPCC; hMLH1; HNPCC2
• NCBI Protein Information: DNA mismatch repair protein Mlh1
• UniProt Protein Name: DNA mismatch repair protein Mlh1
• Protein Family: Putative MutL protein
• UniProt Gene Name: MLH1
• UniProt Synonym Gene Names: COCA2
• UniProt Entry Name: MLH1_HUMAN

NCBI Description

This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.[provided by RefSeq, Nov 2009]

Uniprot Description

MLH1: a protein involved in the repair of mismatches in DNA. Binds PMS2 or MLH1 and MLH3. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50- MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with MBD4. Interacts with EXO1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2; Turcot syndrome, an autosomal dominant disorder characterized by malignant tumors of the brain; Muir-Torre syndrome (MTS), a rare autosomal dominant disorder characterized by sebaceous neoplasms and visceral malignancy; lobular carcinoma in situ; endometrial cancer; and non-polyposis colorectal cancer.

Protein type: Tumor suppressor; DNA repair, damage

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: MutLalpha complex; nucleoplasm; male germ cell nucleus; chiasma; membrane; synaptonemal complex; nucleus

Molecular Function: protein binding; ATPase activity; guanine/thymine mispair binding; MutSalpha complex binding; single-stranded DNA binding; ATP binding

Biological Process: oogenesis; poly(A) tail shortening; meiotic metaphase I plate congression; mismatch repair; DNA damage response, signal transduction resulting in induction of apoptosis; synapsis; somatic hypermutation of immunoglobulin genes; isotype switching; negative regulation of mitotic recombination; male meiosis chromosome segregation; resolution of meiotic joint molecules as recombinants; spermatogenesis; spindle midzone assembly involved in meiosis; DNA repair; double-strand break repair via nonhomologous end joining

Disease: Muir-torre Syndrome; Mismatch Repair Cancer Syndrome; Colorectal Cancer, Hereditary Nonpolyposis, Type 2