Immunohistochemistry (IHC)
(Immunohistochemistry: Formalin-fixed, paraffin-embedded human colon stained with MBS602402 using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epithelial cells.)
Mouse anti-Human Milk Fat Globule Membrane Protein Monoclonal Antibody | anti-Mfge8 antibody
Milk Fat Globule Membrane Protein (HMFG1)
Gene Names
Mfge8; P47; Mfgm; SED1; MFG-E8; AA408458; AI325141; lactadherin; Mfge8
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Milk Fat Globule Membrane Protein; Monoclonal Antibody; Milk Fat Globule Membrane Protein (HMFG1); Anti -Milk Fat Globule Membrane Protein (HMFG1); anti-Mfge8 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
0.N.423
Specificity
Recognizes human milk fat globule membrane protein (HMFG1) at 40-45kD. Does not crossreact with rat.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 15mM sodium azide.
Applicable Applications for anti-Mfge8 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunoprecipitation.
Dilution: Western Blot: 0.5-1ug/ml for 2hrs at RT.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Immunoprecipitation (Native and denatured): 2ug/mg protein lysate. Use Protein G.
Dilution: Western Blot: 0.5-1ug/ml for 2hrs at RT.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Immunoprecipitation (Native and denatured): 2ug/mg protein lysate. Use Protein G.
Immunogen
Delipidated human milk fat globule membrane preparation. Cellular Localization: Cell membrane and cytoplasmic.
Hybridoma
P3-X63-Ag8.653 myeloma cells with spleen cells from BALB/c mice.
Positive Control
SKBR-3 cells. Breast, colon, or lung tissues.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Immunohistochemistry: Formalin-fixed, paraffin-embedded human colon stained with MBS602402 using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epithelial cells.)
Immunohistochemistry (IHC)
(Immunohistochemistry: Formalin-fixed, paraffin-embedded human colon stained with MBS602402 using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epithelial cells.)
Western Blot (WB)
(Immunoprecipitation (native)/Western Blot of human cells using using MBS602402.)
Western Blot (WB)
(Immunoprecipitation (native)/Western Blot of human cells using using MBS602402.)
Western Blot (WB)
(Western Blot of human cells using using MBS602402.)
Western Blot (WB)
(Western Blot of human cells using using MBS602402.)
Related Product Information for anti-Mfge8 antibody
HMFG1 is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG1 is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.
Product Categories/Family for anti-Mfge8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
51,241 Da
NCBI Official Full Name
milk fat globule membrane protein E8
NCBI Official Synonym Full Names
milk fat globule-EGF factor 8 protein
NCBI Official Symbol
Mfge8
NCBI Official Synonym Symbols
P47; Mfgm; SED1; MFG-E8; AA408458; AI325141; lactadherin; Mfge8
NCBI Protein Information
lactadherin; MP47; EGF/factor VIII; sperm surface protein SP47; milk fat globule glycoprotein
UniProt Protein Name
Lactadherin
Protein Family
UniProt Gene Name
Mfge8
UniProt Entry Name
MFGM_MOUSE
Uniprot Description
MFGE8: Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization. Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extrinsic to plasma membrane; extracellular matrix; extracellular space; membrane; extracellular region; vesicle; external side of plasma membrane
Molecular Function: integrin binding; phosphatidylethanolamine binding; phosphatidylserine binding
Biological Process: single fertilization; positive regulation of phagocytosis; phagocytosis, recognition; angiogenesis; phagocytosis, engulfment; cell adhesion
Research Articles on Mfge8
1. MFG-E8 attenuates inflammation and ALI after gut I/R and may represent a novel therapeutic agent.
2. Mfge8 directly bound collagen, and Mfge8-/- macrophages exhibited defective collagen uptake that could be rescued by recombinant Mfge8 containing at least one discoidin domain.
3. Data suggest that milk-fat globule-EGF factor 8 seems to act on the phagocytosis of apoptotic granulosa cells via macrophages and contribute to the regression process of atretic follicles.
4. Recombinant murine MFGE8 is able to reconstitute the clearance of apoptotic cells in septic rats to levels observed in sham operated animals.
5. Performs important role in cell-cell binding, or in promoting phagocytosis.
6. Results suggest that systemic milk fat globule epidermal growth factor-8 blockade might intensify the antitumor activities of existing therapeutic regimens through coordinated cell-autonomous and immune-mediated mechanisms.
7. MFG-E8 altered alpha(v)beta(3) integrin-mediated focal adhesion kinase phosphorylation by impeding the binding of one of its potent ligands osteopontin, which becomes activated during colitis.
8. SED1 facilitates epididymal cell adhesion, and that its loss leads to breakdown of the epididymal epithelium and consequent development of spermatic granulomas.
9. Splenic MFG-E8 production decreases in a time-dependent manner in septic wild-type mice; endotoxemia suppresses MFG-E8 mRNA expression in the spleen in a dose-dependent manner.
10. Splenic macrophages from lactadherin-deficient mice had diminished capacity to phagocytose phosphatidylserine-expressing RBCs.
2. Mfge8 directly bound collagen, and Mfge8-/- macrophages exhibited defective collagen uptake that could be rescued by recombinant Mfge8 containing at least one discoidin domain.
3. Data suggest that milk-fat globule-EGF factor 8 seems to act on the phagocytosis of apoptotic granulosa cells via macrophages and contribute to the regression process of atretic follicles.
4. Recombinant murine MFGE8 is able to reconstitute the clearance of apoptotic cells in septic rats to levels observed in sham operated animals.
5. Performs important role in cell-cell binding, or in promoting phagocytosis.
6. Results suggest that systemic milk fat globule epidermal growth factor-8 blockade might intensify the antitumor activities of existing therapeutic regimens through coordinated cell-autonomous and immune-mediated mechanisms.
7. MFG-E8 altered alpha(v)beta(3) integrin-mediated focal adhesion kinase phosphorylation by impeding the binding of one of its potent ligands osteopontin, which becomes activated during colitis.
8. SED1 facilitates epididymal cell adhesion, and that its loss leads to breakdown of the epididymal epithelium and consequent development of spermatic granulomas.
9. Splenic MFG-E8 production decreases in a time-dependent manner in septic wild-type mice; endotoxemia suppresses MFG-E8 mRNA expression in the spleen in a dose-dependent manner.
10. Splenic macrophages from lactadherin-deficient mice had diminished capacity to phagocytose phosphatidylserine-expressing RBCs.
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Product Notes
The Mfge8 mfge8 (Catalog #AAA602402) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Milk Fat Globule Membrane Protein (HMFG1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Milk Fat Globule Membrane Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunoprecipitation. Dilution: Western Blot: 0.5-1ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Immunoprecipitation (Native and denatured): 2ug/mg protein lysate. Use Protein G. Researchers should empirically determine the suitability of the Mfge8 mfge8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Milk Fat Globule Membrane Protein, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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