Typical Testing Data/Standard Curve (for reference only)
(Mouse MFGE8/Lactadherin PicoKine ELISA Kit standard curve )
Mouse MFGE8/Lactadherin ELISA Kit | MFGE8 elisa kit
Mouse MFGE8/Lactadherin PicoKine ELISA Kit
Gene Names
Mfge8; P47; MP47; Mfgm; SED1; MFG-E8; AA408458; AI325141; lactadherin
Reactivity
Mouse
Synonyms
MFGE8/Lactadherin; Mouse MFGE8/Lactadherin PicoKine ELISA Kit; Lactadherin; milk fat globule-EGF factor 8 protein; BA46; Breast epithelial antigen BA46; Contains Medin; EDIL1; HMFG; hP47; HsT19888; LactadherinLactadherin [Precursor]; Medin; MFG E8; MFG-E8; MFGE8; MFGM; MFGM_HUMAN; Milk fat globule EGF factor 8; Milk fat globule EGF factor 8 protein; Milk fat globule-EGF factor 8; O acetyl disialoganglioside synthase; OAcGD3S; SED1; SPAG10; Sperm associated antigen 10; Sperm surface protein hP47; MFGE8 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse MFGE8
Sequence Length
463
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse MFGE8/Lactadherin PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse MFGE8/Lactadherin PicoKine ELISA Kit standard curve )
Related Product Information for MFGE8 elisa kit
Principle of the assay: mouse MFGE8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MFGE8 has been precoated onto 96-well plates. Standards(NSO, A23-C463) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MFGE8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MFGE8 amount of sample captured in plate.
Background: MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, the MFGE8 gene was mapped to chromosome 15q25. MFGE8 is a factor that links apoptotic cells to phagocytes. It specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. It showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE)-dependent mechanism.
Background: MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, the MFGE8 gene was mapped to chromosome 15q25. MFGE8 is a factor that links apoptotic cells to phagocytes. It specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. It showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE)-dependent mechanism.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
47,169 Da
NCBI Official Full Name
Lactadherin
NCBI Official Synonym Full Names
milk fat globule-EGF factor 8 protein
NCBI Official Symbol
Mfge8
NCBI Official Synonym Symbols
P47; MP47; Mfgm; SED1; MFG-E8; AA408458; AI325141; lactadherin
NCBI Protein Information
lactadherin; EGF/factor VIII; sperm surface protein SP47; milk fat globule glycoprotein
UniProt Protein Name
Lactadherin
Protein Family
UniProt Gene Name
Mfge8
UniProt Synonym Gene Names
MFG-E8; MP47
UniProt Entry Name
MFGM_MOUSE
Uniprot Description
MFGE8: Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization. Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extrinsic to plasma membrane; extracellular matrix; extracellular space; membrane; extracellular region; vesicle; external side of plasma membrane
Molecular Function: integrin binding; phosphatidylethanolamine binding; phosphatidylserine binding
Biological Process: single fertilization; positive regulation of phagocytosis; phagocytosis, recognition; angiogenesis; phagocytosis, engulfment; cell adhesion
Research Articles on MFGE8
Similar Products
Product Notes
The Mouse MFGE8 mfge8 (Catalog #AAA177195) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177195 ELISA Kit recognizes Mouse MFGE8. It is sometimes possible for the material contained within the vial of "MFGE8/Lactadherin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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