Mouse anti-Human MIG/CXCL9 Monoclonal Antibody | anti-CXCL9 antibody

Anti-MIG/CXCL9 Antibody, Mouse Monoclonal

Cat. Num. AAA8104249

• Gene Names: CXCL9; CMK; MIG; Humig; SCYB9; crg-10
• Reactivity: Human
• Applications: ELISA, ELISA
• Purity: Protein A
• Synonyms: MIG/CXCL9; Monoclonal Antibody; Anti-MIG/CXCL9 Antibody; Mouse Monoclonal; CXCL9/MIG Antibody; Mouse MAb; chemokine (C-X-C motif) ligand 9; Anti-CMK Antibody; Anti-crg-10 Antibody; Anti-Humig Antibody; Anti-MIG Antibody; Anti-SCYB9 Antibody; anti-CXCL9 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: 06
• Specificity: Human CXCL9/MIG.; No cross-reactivity in ELISA with Mouse CXCL9 E Coli cell lysate
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CXCL9 antibody: ELISA (EIA), ELISA (EIA) (Cap)
• Application Notes: ELISA: 1:1000-1:2000; ELISA (Cap): 1:250-1:2000; ELISA (Cap): This antibody will detect Human CXCL9/MIG in ELISA pair set.; In a sandwich ELISA, it can be used as capture antibody when paired with.
• Immunogen: Recombinant Human CXCL9/MIG protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CXCL9/MIG (rh CXCL9/MIG). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-CXCL9 antibody

Chemokine (C-X-C motif) ligand 9 (CXCL9), also known as Monokine induced by gamma interferon (MIG), is a small cytokine belonging to the CXC chemokine family. The function of this chemokine has not been specifically defined; however, it is thought to be involved in T cell trafficking. CXCL9/MIG functions as one of the three ligands of chemokine receptor CXCR3 which is a G protein-coupled receptor found predominantly on T cells. CXCL9/MIG, together with CXCL10 and CXCL11, may activate CXCR3 by binding to it. CXCL9 serves as a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. It has been observed that tumour endothelial cells secrete high levels of CXCL9 in all, and CXCL10 in most melanoma metastases. Experiment data represent novel mechanisms by which tumour cells in melanoma metastases might use the chemokine-expressing endothelium to leave the tumour and eventually to form additional metastases at distinct sites. Experiment results also improved that CXCL9/MIG plays an important role in CD4+ T lymphocyte recruitment and development of CAV, MOMA-2+ macrophages are the predominant recipient-derived source of CXCL9/MIG, and recipient CD4 lymphocytes are necessary for sustained CXCL9/MIG production and CAV development in this model. Neutralization of the chemokine CXCL9/MIG may have therapeutic potential for the treatment of chronic rejection after heart transplantation.

References

Ruehlmann JM, et al. (2001) MIG (CXCL9) chemokine gene therapy combines with antibody-cytokine fusion protein to suppress growth and dissemination of murine colon carcinoma. Cancer Res. 61(23): 8498-503.Belperio JA, et al. (2003) Role of CXCL9/CXCR3 chemokine biology during pathogenesis of acute lung allograft rejection. J Immunol. 171(9): 4844-52.Colvin RA, et al. (2004) Intracellular domains of CXCR3 that mediate CXCL9, CXCL10, and CXCL11 function. J Biol Chem. 279(29): 30219-27.Valbuena G, et al. (2003) Expression analysis of the T-cell-targeting chemokines CXCL9 and CXCL10 in mice and humans with endothelial infections caused by rickettsiae of the spotted fever group. Am J Pathol. 163(4): 1357-69.

NCBI and Uniprot Product Information

• NCBI GI #: 4505187
• NCBI GeneID: 4283
• NCBI Accession #: NP_002407.1
• NCBI GenBank Nucleotide #: NM_002416.2
• UniProt Accession #: Q07325; Q503B4
• Molecular Weight: 14,019 Da
• NCBI Official Full Name: C-X-C motif chemokine 9
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 9
• NCBI Official Symbol: CXCL9
• NCBI Official Synonym Symbols: CMK; MIG; Humig; SCYB9; crg-10
• NCBI Protein Information: C-X-C motif chemokine 9; gamma-interferon-induced monokine; monokine induced by gamma interferon; monokine induced by interferon-gamma; small-inducible cytokine B9
• UniProt Protein Name: C-X-C motif chemokine 9
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: CXCL9
• UniProt Synonym Gene Names: CMK; MIG; SCYB9; HuMIG; MIG
• UniProt Entry Name: CXCL9_HUMAN

NCBI Description

This antimicrobial gene encodes a protein thought to be involved in T cell trafficking. The encoded protein binds to C-X-C motif chemokine 3 and is a chemoattractant for lymphocytes but not for neutrophils. [provided by RefSeq, Sep 2014]

Uniprot Description

CXCL9: Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: protein binding; CXCR3 chemokine receptor binding; chemokine activity; cytokine activity

Biological Process: defense response; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of cAMP metabolic process; chemotaxis; signal transduction; positive regulation of myoblast differentiation; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; cell-cell signaling; cellular defense response; immune response; positive regulation of release of sequestered calcium ion into cytosol; inflammatory response; defense response to virus